Relative gene expression in hippocampus
| Gene | Mean ± SEM | Kolmogorov-Smirnov test for normality (p value) | Statistical significance |
|---|---|---|---|
| BACE1 | E3FAD CTL = 1 ± N/A E3FAD WD = 1.53 ± 0.31 E4FAD CTL = 1.32 ± 0.19 E4FAD WD = 1.76 ± 0.41 | E3FAD CTL > 0.10 E3FAD WD > 0.10 E4FAD CTL > 0.10 E4FAD WD > 0.10 | Genotype: F(1,28) = 1.10, p = 0.304 diet: F(1,28) = 3.44, p = 0.074 interaction: F(1,28) = 0.03, p = 0.874 |
| Neprilysin | E3FAD CTL = 1 ± N/A E3FAD WD = 1.61 ± 0.79 E4FAD CTL = 0.94 ± 0.30 E4FAD WD = 1.79 ± 0.63 | E3FAD CTL > 0.10 E3FAD WD > 0.10 E4FAD CTL > 0.10 E4FAD WD > 0.10 | Genotype: F(1,28) = 0.02, p = 0.902 diet: F(1,28) = 2.49, p = 0.126 interaction: F(1,28) = 0.06, p = 0.802 |
| IDE | E3FAD CTL = 1 ± N/A E3FAD WD = 1.27 ± 0.39 E4FAD CTL = 1.30 ± 0.39 E4FAD WD = 1.12 ± 0.35 | E3FAD CTL > 0.10 E3FAD WD > 0.10 E4FAD CTL = 0.01 E4FAD WD > 0.10 | Genotype: F(1,28) = 0.08, p = 0.785 diet: F(1,28) = 0.00, p = 0.955 interaction: F(1,28) = 0.49, p = 0.489 |
| CD68 | E3FAD CTL = 1 ± N/A E3FAD WD = 1.21 ± 0.29 E4FAD CTL = 1.74 ± 0.30 E4FAD WD = 2.30 ± 0.29 | E3FAD CTL > 0.10 E3FAD WD > 0.10 E4FAD CTL > 0.10 E4FAD WD > 0.10 | Genotype: F(1,28) = 10.75, p = 0.003 diet: F(1,28) = 1.91, p = 0.178 interaction: F(1,28) = 0.40, p = 0.532 |
| GFAP | E3FAD CTL = 1 ± N/A E3FAD WD = 1.02 ± 0.11 E4FAD CTL = 1.56 ± 0.21 E4FAD WD = 2.70 ± 0.04 | E3FAD CTL > 0.10 E3FAD WD > 0.10 E4FAD CTL > 0.10 E4FAD WD > 0.10 | Genotype: F(1,28) = 14.26, p < 0.001 diet: F(1,28) = 0.23, p = 0.634 interaction: F(1,28) = 0.14, p = 0.712 |
| CD74 | E3FAD CTL = 1 ± N/A E3FAD WD = 1.28 ± 0.28 E4FAD CTL = 3.32 ± 0.62 E4FAD WD = 5.04 ± 1.30 | E3FAD CTL > 0.10 E3FAD WD = 0.01 E4FAD CTL > 0.10 E4FAD WD > 0.10 | Genotype: F(1,28) = 16.98, p < 0.001 diet: F(1,28) = 1.86, p = 0.184 interaction: F(1,28) = 0.96, p = 0.335 |
Data are presented as mean fold differences (±SEM) relative to E3FAD mice on a control diet. The Kolmogorov-Smirnov test for normality was performed, with p > 0.05 indicating a normal distribution. Genes related to β-amyloid production (BACE-1) and clearance (neprilysin, IDE) showed no significant changes with either diet or genotype, while genes related to glial activation (CD68, GFAP, and CD74) were increased in E4FAD mice on both control and Western diets.