Statistical analyses performed for each experiment
| Figure/panel | Assay | Statistical test | F(df, error value), p value | Post hoc analysis/correction |
|---|---|---|---|---|
| 2A | Training for short-term memory | Two-way repeated measures ANOVA | Interaction: F(1,12) = 3.446, p = 0.0881Time: F(1,12) = 42.49, p < 0.0001Experimental group: F(1,12) = 1.686, p = 0.2185 | Holm-Sidak's multiple comparisons test |
| 2B | Training for long-term memory | Two-way repeated measures ANOVA | Interaction: F(1,16) = 1.986, p = 0.1779Time: F(1,16) = 88.27, p < 0.0001Experimental group: F(1,16) = 0.4603, p = 0.5072 | Holm-Sidak's multiple comparisons test |
| 3C, left | Cued short-term memory pre CS | Ordinary one-way ANOVA | F(3,46) = 16.69, p = 0.0100 | Holm-Sidak's multiple comparisons test |
| 3C, right | Cued short-term memory CS | Ordinary one-way ANOVA | F(3,43) = 16.69, p < 0.0001 | Holm-Sidak's multiple comparisons test |
| 3D | Contextual long-term memory | Ordinary one-way ANOVA | F(3,46) = 16.72, p < 0.0001 | Holm-Sidak's multiple comparisons test |
| 3E, left | Cued long-term memory pre CS | Ordinary one-way ANOVA | F(3,46) = 16.69, p = 0.0100 | Holm-Sidak's multiple comparisons test |
| 3E, right | Cued long-term memory CS | Ordinary one-way ANOVA | F(3,45) = 20.39, p < 0.0001 | Holm-Sidak's multiple comparisons test |
| 3F | Western blotting of P-S6 | Ordinary one-way ANOVA | F(3,13) = 9.391, p = 0.0014 | Holm-Sidak's multiple comparisons test |
| 3G | Western blotting of P-Akt | Ordinary one-way ANOVA | F(3,11) = 5.058, p = 0.0192 | Holm-Sidak's multiple comparisons test |
| 4B | Protrusions per micrometer | Ordinary one-way ANOVA | F(3,613) = 13.55, p < 0.0001 | Holm-Sidak's multiple comparisons test |
| 4C | LWR | Ordinary one-way ANOVA | F(3,645) = 14.45, p = 0.0009 | Holm-Sidak's multiple comparisons test |
| 4D | Immature spines per micrometer | Ordinary one-way ANOVA | F(3,609) = 4.505, p = 0.0039 | Holm-Sidak's multiple comparisons test |
| 4E | Mature spines per micrometer | Ordinary one-way ANOVA | F(3,613) = 4.845, p = 0.0023 | Holm-Sidak's multiple comparisons test |
| 5B, left | Contextual long-term memory 16-h training | Unpaired Student's t test | t(18.31) = 0.3066, p = 0.7626 | Welch's correction |
| 5B, right | Cued long-term memory | Two-way repeated measures ANOVA | Interaction: F(1,21) = 0.5235, p = 0.4773Time: F(1,21) = 133.2, p < 0.0001Experimental group: F(1,21) = 0.3028, p = 0.5879 | Holm-Sidak's multiple comparisons test |
| 5C, right | Western blotting of P-S6 and P-Akt | Unpaired Student's t test (for S6 only) | t(6.257) = 0.5123, p = 0.6260 | Welch's correction |
| Unpaired Student's t test (for AKT only) | t(7.997) = 1.713, p = 0.1250 | Welch's correction |