Quantification of rabies-labeled presynaptic inputs to hilar mossy cells
| A. Individual case summary | ||||||||||
| Starter neurons | DG GCL | DG ML | DG Hilus | CA3c SP | CA3c non-SP | CA3b SP | EC | MS-DB | SFi | |
|---|---|---|---|---|---|---|---|---|---|---|
| Case 1561 (total 365 neuron counts) | ||||||||||
| Number of neurons | 36 | 227 | 15 | 59 | 16 | 12 | 5 | 0 | 17 | 10 |
| CSI | 6.31 | 0.42 | 1.64 | 0.44 | 0.33 | 0.14 | 0.00 | 0.47 | 0.28 | |
| % of total labeling | 62.19 | 4.11 | 16.16 | 4.38 | 3.29 | 1.37 | 0.00 | 4.66 | 2.74 | |
| Case 1562 (total 431 neuron counts) | ||||||||||
| Number of neurons | 39 | 262 | 15 | 79 | 22 | 15 | 4 | 1 | 12 | 11 |
| CSI | 6.72 | 0.38 | 2.03 | 0.56 | 0.38 | 0.10 | 0.03 | 0.31 | 0.28 | |
| % of total labeling | 60.79 | 3.48 | 18.33 | 5.10 | 3.48 | 0.93 | 0.23 | 2.78 | 2.55 | |
| Case 2543 (total 1247 neuron counts) | ||||||||||
| Number of neurons | 70 | 660 | 33 | 366 | 78 | 20 | 21 | 0 | 20 | 3 |
| CSI | 9.43 | 0.47 | 5.23 | 1.11 | 0.29 | 0.30 | 0.00 | 0.29 | 0.04 | |
| % of total labeling | 52.93 | 2.65 | 29.35 | 6.26 | 1.60 | 1.68 | 0.00 | 1.60 | 0.24 | |
| Case 2544 (total 1333 neuron counts) | ||||||||||
| Number of neurons | 62 | 702 | 59 | 369 | 49 | 22 | 27 | 2 | 31 | 6 |
| CSI | 11.32 | 0.95 | 5.95 | 0.79 | 0.35 | 0.44 | 0.03 | 0.50 | 0.10 | |
| % of total labeling | 52.66 | 4.43 | 27.68 | 3.68 | 1.65 | 2.03 | 0.15 | 2.33 | 0.45 | |
| B. Summary data of all 4 cases | |||||||||
| DG GCL | DG ML | DG Hilus | CA3c SP | CA3c non-SP | CA3b SP | EC | MS-DB | SFi | |
| CSI | 8.44 | 0.56 | 3.71 | 0.73 | 0.34 | 0.24 | 0.01 | 0.39 | 0.17 |
| SE | 1.18 | 0.13 | 1.10 | 0.15 | 0.02 | 0.08 | 0.01 | 0.06 | 0.06 |
| % of total labeling | 57.14 | 3.67 | 22.88 | 4.85 | 2.51 | 1.50 | 0.10 | 2.84 | 1.50 |
| SE | 2.53 | 0.39 | 3.30 | 0.55 | 0.51 | 0.23 | 0.06 | 0.65 | 0.67 |
As every one in three sections across the whole brain series was used for mapping rabies-labeled presynaptic neurons of targeted dentate granule cells, the actual total number of labeled neurons in each case would be estimated three times of the total neuron counts listed in the table. The labeled neurons are predominantly distributed in the ipsilateral hemisphere. The neuronal count for each structure in each case is pooled from both hemispheres if there are any labeled neurons in the contralateral hemisphere. The percentage of total labeled neurons is calculated as the percentage of the number of labeled presynaptic neurons in a specified structure versus the overall total presynaptic neuron count in each case. The input CSI is defined as the ratio of the number of labeled presynaptic neurons in a specified structure versus the number of starter neurons.
DG GCL, DG granule cell layer; DG ML, DG molecular layer; CA3b-c SP, CA3b-c pyramidal cell layer; CA3c non-SP, CA3c nonpyramidal cell layer.