Static intrinsic properties of MVN neurons.
| All | type A | type B | Statistical tests (Mann–Whitney) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Property | Control | VVM | Control | VVM | Control | VVM | Control vs. VVM | |||||
| Number of neurons | 63 | 60 | 27 | 17 | 36 | 43 | All | type A | type B | |||
| Membrane potential (mV) | –48.28 | –49.00 | –47.24 | –47.22 | –49.07 | –49.70 | z = –1.09 | NS | z = –0.02 | NS | z = –0.81 | NS |
| Spike threshold (mV) | –31.32 | –32.34 | –29.58 | –30.73 | –32.63 | –32.97 | z = –1.36 | NS | z = –0.99 | NS | z = –0.39 | NS |
| Firing rate (Hz) | 12.24 | 7.85 | 13.83 | 8.14 | 11.04 | 7.74 | z = –2.49 | p = 0.013 | z = –1.73 | p = 0.08 | z = –1.57 | NS |
| Coefficient of variation | 0.16 | 0.22 | 0.15 | 0.22 | 0.17 | 0.22 | z = 1.97 | p = 0.048 | z = 1.40 | NS | z = 1.11 | NS |
| AHPR (V/s) | 0.20 | 0.21 | 0.44 | 0.67 | 0.02 | 0.03 | z = –1.29 | NS | z = –0.09 | NS | z = –0.11 | NS |
| dAHP (V/s) | 0.71 | 0.69 | 0.00 | 0.00 | 1.24 | 0.96 | z = –0.07 | NS | NA | NA | z = –1.14 | NS |
| AHP (mV) | 26.11 | 25.25 | 29.68 | 28.54 | 23.43 | 23.95 | z = –0.16 | NS | z = –0.14 | NS | z = 0.48 | NS |
| Concavity (mV) | –1.33 | –1.50 | –2.49 | –3.47 | –0.46 | –0.73 | z = 0.002 | NS | z = –1.49 | NS | z = –0.59 | NS |
| Convexity (mV) | 0.82 | 0.84 | 0.56 | 0.34 | 1.02 | 1.05 | z = 0.13 | NS | z = –0.98 | NS | z = 0.03 | NS |
Parameters of the spontaneous activity of MVN neurons recorded on control and VVM slices. Static parameters are based on the analysis of the pacemaker discharge of the neurons and the quantification of the AHP and interspike interval. Neurons are pooled (all) or segmented in type A and type B. Overall, the intrinsic membrane properties recorded in controls and VVM mice show limited differences between type A and type B neurons. AHPR, dAHP, and AHP are parameters representing kinetics of the AHP and interspike interval (see Materials and methods). Statistically significant differences are highlighted in red. NS, not significant.