Table 1:

Conductivities in ACSF and brain tissue following different current injection conditions

ACSF_R conductivity (S/m)
	Current amplitude, n = 6			Electrode distance, n = 6
Frequency	175 pA	300 pA	500 pA	100 µm	125 µm
5 Hz	1.03 ± 0.02	1.24 ± 0.00	1.16 ± 0.00	1.07± 0.14	1.23 ± 0.03
60 Hz	1.29 ± 0.02	1.26 ± 0.03	1.27 ± 0.01	1.23 ± 0.01	1.27± 0.02
100 Hz	1.23 ± 0.05	1.30 ± 0.02	1.27 ± 0.01	1.29 ± 0.01	1.24 ± 0.03
300 Hz	1.36 ± 0.04	1.42 ± 0.02	1.39 ± 0.03	1.42 ± 0.01	1.36 ± 0.03
500 Hz	1.55 ± 0.06	1.60 ± 0.03	1.56 ± 0.00	1.60 ± 0.02	1.54± 0.03
ACSF_c conductivity (S/m)
	Current amplitude, n = 12			Electrode distance, n = 12
Frequency	175 pA	300 pA	500 pA	100 µm	125 µm
5Hz	0.46 ± 0.05	0.49 ± 0.04	0.48 ± 0.02	0.49 ± 0.03	0.46 ± 0.03
60Hz	0.52 ± 0.02	0.51 ± 0.02	0.50 ± 0.02	0.51 ± 0.02	0.52 ± 0.02
100Hz	0.55 ± 0.03	0.53 ± 0.02	0.53 ± 0.02	0.52 ± 0.02	0.55 ± 0.02
300Hz	0.59 ± 0.03	0.60 ± 0.03	0.59 ± 0.04	0.57 ± 0.03	0.61 ± 0.02
500Hz	0.63 ± 0.04	0.64 ± 0.05	0.63 ± 0.05	0.62 ± 0.03	0.65 ± 0.03
Brain slice conductivity (S/m)
	Current amplitude, n = 54			Electrode distance, n = 54
Frequency	175 pA	300 pA	500 pA	100 µm	125 µm
5 Hz	0.37 ± 0.03	0.37 ± 0.04	0.38 ± 0.04	0.37 ± 0.03	0.38 ± 0.03
60 Hz	0.40 ± 0.04	0.41 ± 0.04	0.42 ± 0.04	0.41 ± 0.04	0.41 ± 0.03
100 Hz	0.43 ± 0.05	0.42 ± 0.05	0.43 ± 0.05	0.42 ± 0.04	0.43 ± 0.04
300 Hz	0.48 ± 0.05	0.47 ± 0.05	0.47 ± 0.05	0.47 ± 0.04	0.48 ± 0.04
500 Hz	0.55 ± 0.05	0.54 ± 0.05	0.54 ± 0.05	0.53 ± 0.04	0.56 ± 0.04

Current amplitude and electrode distance do not affect the frequency-dependent increase in conductivity. Groups were compared using two-way ANOVA. Overall test statistics: ACSF_R: amplitude, F₍₈₎ = 0.45, p = 0.88; distance, F₍₄₎ = 2.15, p = 0.11; ACSF_C: amplitude, F₍₈₎ = 0.06, p = 0.99; distance, F₍₄₎ = 0.57, p = 0.68; brain slice amplitude: amplitude, F₍₈₎ = 0.02, p = 1.00; distance, F₍₄₎ = 0.14, p = 0.96.