Summary of statistical analysis.
| Line | Data analyzed | Results | Data structure | Type of test | Observed power or confidence interval |
|---|---|---|---|---|---|
| a | Total play: female vehicle, female ACEA, female GP1a | F(2, 224) = 0.28, p = 0.756 | Normal | ANOVA | 0.094 |
| b | Pouncing: female vehicle, female ACEA, female GP1a | F(2, 222) = 0.2.713, p = 0.069 | Normal | ANOVA | 0.533 |
| c | Pinning: female vehicle, female ACEA, female GP1a | F(2, 210) = 0.699, p = 0.498 | Normal | ANOVA | 0.167 |
| d | Number of gridlines crossed: female vehicle, female ACEA, female GP1a | F(2, 23) = 1.008, p = 0.383 | Normal | ANOVA | 0.201 |
| e | Time spent in the center zone: female vehicle, female ACEA, female GP1a | F(2, 23) = 0.262, p = 0.772 | Normal | ANOVA | 0.086 |
| f | Total play: male vehicle, male AM281, male AM630 | F(2, 208) = 1.314, p = 0.271 | Normal | ANOVA | 0.282 |
| g | Pouncing: male vehicle, male AM281, male AM630 | F(2, 208) = 2.103, p = 0.125 | Normal | ANOVA | 0.429 |
| h | Pinning: male vehicle, male AM281, male AM630 | F(2, 187) = 5.483, p = 0.005 | Normal | ANOVA | 0.845 |
| i | Pinning: male vehicle, male AM281 | p = 0.675 | Tukey post hoc | –0.4573 to 0.2162 | |
| j | Pinning: male vehicle, male AM630 | p = 0.004 | Tukey post hoc | –0.7868 to –0.1227 | |
| k | Number of gridlines crossed: male vehicle, male AM281, male AM630 | F(2, 21) = 1.150, p = 0.339 | Normal | ANOVA | 0.221 |
| l | Time spent in the center zone: male vehicle, male AM281, male AM630 | F(2, 21) = 1.568, p = 0.236 | Normal | ANOVA | 0.289 |
| m | Total play: male vehicle, female vehicle, male WIN, female WIN, male ACEA+GP1a, female ACEA+GP1a | F(2, 360) = 4.449, p = 0.012 | Normal | 2-Way ANOVA: sex × treatment interaction | 0.762 |
| n | F(1, 360) = 4.108, p = 0.043 | Main effect of sex | 0.524 | ||
| 0 | F(1, 360) = 2.275, p = 0.104 | Main effect of treatment | 0.461 | ||
| p | Total play: male vehicle, female vehicle | t(109.2) = 2.666, p = 0.0089 | t-test | 0.5331 to 3.624 | |
| q | Total play: female vehicle, female WIN | t(127.3) = 2.01, p = 0.0465 | t-test | 0.023 to 2.919 | |
| r | Total play: female vehicle, female ACEA+GP1a | t(114.7) = 2.071, p = 0.0406 | t-test | 0.07583 to 3.415 | |
| s | Total play: male vehicle, female WIN | t(112.5) = 0.7378, p = 0.4622 | t-test | –1.024 to 2.24 | |
| t | Total play: male vehicle, female ACEA+GP1a | t(116.8) = 0.3607, p = 0.719 | t-test | –1.497 to 2.163 | |
| u | Total play: male vehicle, male WIN | t(83.32) = 0.882, p = 0.3803 | t-test | –1.182 to 3.066 | |
| v | Total play: male vehicle, male ACEA+GP1a | t(109) = 1.711, p = 0.0899 | t-test | –3.2 to 0.2348 | |
| w | Pouncing: male vehicle, female vehicle, male WIN, female WIN, male ACEA+GP1a, female ACEA+GP1a | F(2, 359) = 6.914, p = 0.001 | Normal | 2-Way ANOVA: sex × treatment interaction | 0.923 |
| × | F(1, 359) = 3.474, p = 0.63 | Main effect of sex | 0.46 | ||
| y | F(2, 359) = 3.7, p = 0.026 | Main effect of treatment | 0.677 | ||
| z | Pouncing: male vehicle, female vehicle | t(118.7) = 2.347, p = 0.0206 | t-test | 0.2161 to 2.551 | |
| aa | Pouncing: female vehicle, female WIN | t(131) = 1.358, p = 0.1767 | t-test | –0.3653 to 1.966 | |
| bb | Pouncing: female vehicle, female ACEA+GP1a | t(119.8) = 2.123, p = 0.0358 | t-test | 0.09501 to 2.724 | |
| cc | Pouncing: male vehicle, female WIN | t(116.3) = 0.947, p = 0.3456 | t-test | –0.6366 to 1.803 | |
| dd | Pouncing: male vehicle, female ACEA+GP1a | t(115.3) = 0.038, p = 0.9698 | t-test | –1.388 to 1.336 | |
| ee | Pouncing: male vehicle, male WIN | t(77.85) = 1.917, p = 0.0589 | t-test | –0.06252 to 3.323 | |
| ff | Pouncing: male vehicle, male ACEA+GP1a | t(108.9) = 2.069, p = 0.0409 | t-test | –2.554 to –0.05488 | |
| gg | Pinning: male vehicle, female vehicle, male WIN, female WIN, male ACEA+GP1a, female ACEA+GP1a | F(2, 329) = 3.171, p = 0.043 | Normal | 2-Way ANOVA: sex × treatment interaction | 0.605 |
| hh | F(1, 329) = 4.158, p = 0.042 | Main effect of sex | 0.529 | ||
| ii | F(2, 329) = 3.53, p = 0.03 | Main effect of treatment | 0.655 | ||
| jj | Pinning: male vehicle, female vehicle | t(70.79) = 3.206, p = 0.002 | t-test | 0.2814 to 1.207 | |
| kk | Pinning: female vehicle, female WIN | t(99.25) = 5.11, p < 0.0001 | t-test | 0.5536 to 1.256 | |
| ll | Pinning: female vehicle, female ACEA+GP1a | t(89.6) = 2.911, p = 0.0045 | t-test | 0.1803 to 0.9554 | |
| mm | Pinning: male vehicle, female WIN | t(96.71) = 0.6144, p = 0.5404 | t-test | –0.6797 to 0.3584 | |
| nn | Pinning: male vehicle, female ACEA+GP1a | t(103.2) = 0.6438, p = 0.5211 | t-test | –0.367 to 0.7198 | |
| oo | Pinning: male vehicle, male WIN | t(94.97) = 0.002952, p = 0.9977 | t-test | –0.576 to 0.5777 | |
| pp | Pinning: male vehicle, male ACEA+GP1a | t(105) = 0.4127, p = 0.6807 | t-test | –0.4773 to 0.7281 | |
| Number of gridlines crossed: male vehicle, female vehicle, male WIN, female WIN, male ACEA+GP1a, female ACEA+GP1a | F(2, 45) = 2.497, p = 0.095 | Normal | 2-Way ANOVA: sex × treatment interaction | 0.471 | |
| rr | F(2, 45) = 0.39, p = 0.536 | Main effect of sex | 0.094 | ||
| ss | F(1, 45) = 1.145, p = 0.329 | Main effect of treatment | 0.237 | ||
| tt | Time spent in the center zone: male vehicle, female vehicle, male WIN, female WIN, male ACEA+GP1a, female ACEA+GP1a | F(2, 45) = 0.701, p = 0.502 | Normal | 2-Way ANOVA: sex × treatment interaction | 0.16 |
| uu | F(1, 45) = 1.592, p = 0.215 | Main effect of sex | 0.234 | ||
| vv | F(2, 45) = 0.936, p = 0.401 | Main effect of treatment | 0.2 | ||
| ww | Total play: male vehicle, female vehicle, male AM281+AM630, female AM281+AM630 | F(1,240) = 7.081, p = 0.008 | Normal | 2-Way ANOVA: sex × treatment interaction | 0.755 |
| xx | F(1, 240) = 11.592, p = 0.001 | Main effect of sex | 0.924 | ||
| yy | F(1, 240) = 5.207, p = 0.023 | Main effect of treatment | 0.623 | ||
| zz | Total play: male vehicle, female vehicle | t(126) = 4.011, p = 0.0001 | t-test | –9.707 to –3.293 | |
| aaa | Total play: male vehicle, male AM281+AM630 | t(105.5) = 3.195, p = 0.0018 | t-test | –8.584 to –2.01 | |
| bbb | Total play: female vehicle, male AM281+AM630 | t(106.1) = 0.7202, p = 0.473 | t-test | –4.515 to 2.109 | |
| ccc | Total play: female vehicle, female AM281+AM630 | t(106.2) = 0.2976, p = 7.666 | t-test | –2.3 to 3.113 | |
| ddd | Pouncing: male vehicle, female vehicle, male AM281+AM630, female AM281+AM630 | F(1, 240) = 0.7072, p = 0.008 | Normal | 2-Way ANOVA: sex × treatment interaction | 0.754 |
| eee | F(1, 240) = 13.742, p < 0.0001 | Main effect of sex | 0.958 | ||
| fff | F(1, 240) = 6.151, p = 0.014 | Main effect of treatment | 0.695 | ||
| ggg | Pouncing: male vehicle, female vehicle | t(124,9) = 4.191, p < 0.0001 | t-test | –7.361 to –2.639 | |
| hhh | Pouncing: male vehicle, male AM281+AM630 | t(107.5) = 3.225, p = 0.0017 | t-test | –4.036 to 1.252 | |
| iii | Pouncing: female vehicle, male AM281+AM630 | t(103,6) = 0.8051, p = 0.4226 | t-test | –3.337 to 1.41 | |
| jjj | Pouncing: female vehicle, female AM281+AM630 | t(107.8) = 0.1472, p = 0.8833 | t-test | –1.754 to 2.035 | |
| kkk | Pinning: male vehicle, female vehicle, male AM281+AM630, female AM281+AM630 | F(1, 235) = 10.073, p = 0.002 | Normal | 2-Way ANOVA: sex × treatment interaction | 0.885 |
| lll | F(1, 235) = 1.538, p = 0.216 | Main effect of sex | 0.235 | ||
| mmm | F(1, 235) = 2.716, p = 0.101 | Main effect of treatment | 0.375 | ||
| nnn | Pinning: male vehicle, female vehicle | t(122.1) = 3.015, p = 0.0031 | t-test | –2.532 to –0.5248 | |
| ooo | Pinning: male vehicle, male AM281+AM630 | t(106.5) = 3.251, p = 0.0015 | t-test | –2.687 to –0.6514 | |
| ppp | Pinning: female vehicle, male AM281+AM630 | t(101.2) = 0.2961, p = 0.7678 | t-test | –0.8049 to 1.087 | |
| qqq | Pinning: female vehicle, female AM281+AM630 | t(121.8) = 1.189, p = 0.2368 | t-test | –0.3514 to 1.408 | |
| rrr | Number of gridlines crossed: male vehicle, female vehicle, male AM281+AM630, female AM281+AM630 | F(1, 32) = 0.119, p = 0.733 | Normal | 2-Way ANOVA: sex × treatment interaction | 0.063 |
| sss | F(1, 32) = 0.001, p = 0.872 | Main effect of sex | 0.05 | ||
| ttt | F(1, 32) = 0.071, p = 0.792 | Main effect of treatment | 0.058 | ||
| uuu | Time spent in the center zone: male vehicle, female vehicle, male AM281+AM630, female AM281+AM630 | F(1, 32) = 0.377, p - 0.544 | Normal | 2-Way ANOVA: sex × treatment interaction | 0.091 |
| vvv | F(1, 32) = 0.008, p = 0.93 | Main effect of sex | 0.051 | ||
| www | F(1, 32) = 0.406, p = 0.529 | Main effect of treatment | 0.094 | ||
| xxx | PN4 cell body area, left hemisphere: male, female | t(56.06) = 0.0325, p = 0.0325 | Normal | t-test | –311.4 to –14.03 |
| yyy | PN4 cell body area, right hemisphere: male, female | t(48.57) = 2.088, p = 0.042 | Normal | t-test | –353 to –6.739 |
| zzz | PN4 number of dendrites, left hemisphere: male, female | t(73.5) = 0.9388, p = 0.3509 | Normal | t-test | –1.024 to 0.368 |
| aaaa | PN4 number of dendrites, right hemisphere: male, female | t(68.86) = 1.385, p = 0.1705 | Normal | t-test | –0.2165 to 1.2 |
| bbbb | PN4 number of nodes, left hemisphere: male, female | t(73.09) = 0.9446, p = 0.348 | Normal | t-test | –1.935 to 0.6905 |
| cccc | PN4 number of nodes, right hemisphere: male, female | t(71.89) = 0.643, p = 0.5223 | Normal | t-test | –0.5776 to 1.128 |
| dddd | PN4 spine density, left hemisphere: male, female | t(72.17) = 0.2635, p = 0.7929 | Normal | t-test | –0.01184 to 0.01545 |
| eeee | PN4 spine density, right hemisphere: male, female | t(72.52) = 0.06693, p = 0.9468 | Normal | t-test | –0.01593 to 0.01489 |
| ffff | PN4 total dendrite length, left hemisphere: male, female | t(74) = 1.527, p = 0.1309 | Normal | t-test | –64.47 to 8.518 |
| gggg | PN4 total dendrite length, right hemisphere: male, female | t(72.09) = 0.2125, p = 0.8323 | Normal | t-test | –28.12 to 34.83 |
| hhhh | PN4 average dendrite length, left hemisphere: male, female | t(71.66) = 1.452, p = 0.1509 | Normal | t-test | –14.13 to 2.221 |
| iiii | PN4 average dendrite length, right hemisphere: male, female | t(71.1) = 0.82898, p = 0.4094 | Normal | t-test | –11.26 to 4.641 |
| PN26 factor analysis: sex differences in neuronal morphology | |||||
| jjjj | Factor 1, left hemisphere: male, female | t(40.31) = 0.4664, p = 0.6435 | Normal | t-test | –0.4694 to 0.7511 |
| kkkk | Factor 1, right hemisphere: male, female | t(28.31) = 3.454, p = 0.0018 | Normal | t-test | –1.479 to –0.378 |
| llll | Factor 2, left hemisphere: male, female | t(43.65) = 0.2945, p = 0.7698 | Normal | t-test | –0.6725 to 0.5011 |
| mmmm | Factor 2, right hemisphere: male, female | t(30.22) = 0.7176, p = 0.4785 | Normal | t-test | –0.4041 to 0.842 |
| nnnn | Factor 3, left hemisphere: male, female | t(42.96) = 0.9127, p = 0.3665 | Normal | t-test | –0.3008 to 0.798 |
| oooo | Factor 3, right hemisphere: male, female | t(46) = 1.431, p = 0.1592 | Normal | t-test | –0.9151 to 0.1546 |
| PN26 factor analysis: effects of specific and dual agonism on neuronal morphology | |||||
| pppp | Factor 1, left hemisphere: female vehicle, female ACEA, female GP1a | F(2, 60) = 0.533, p = 0.0589 | Normal | ANOVA | 0.134 |
| qqqq | Factor 1, left hemisphere, female vehicle: female ACEA+GP1a | t(38.27) = 0.4245, p = 0.6735 | Normal | t-test | –0.4879 to 0.747 |
| rrrr | Factor 1, right hemisphere: female vehicle, female ACEA, female GP1a | F(2, 60) = 2.099, p = 0.132 | Normal | ANOVA | 0.414 |
| ssss | Factor 1, right hemisphere: female vehicle, female ACEA+GP1a | t(37.95) = 1.639, p = 0.1094 | Normal | t-test | –1.193 to 0.1255 |
| tttt | Factor 2, left hemisphere: female vehicle, female ACEA, female GP1a | F(2, 60) = 2.576, p = 0.085 | Normal | ANOVA | 0.494 |
| left hemisphere: female vehicle, female ACEA, female GP1a | |||||
| uuuu | Factor 2, left hemisphere: female vehicle, female ACEA+GP1a | t(36.6) = 0.2349, p = 0.8156 | Normal | t-test | –0.5315 to 0.6708 |
| vvvv | Factor 2, right hemisphere: female vehicle, female ACEA, female GP1a | F(2, 60) = 0.235, p = 0.791 | Normal | ANOVA | 0.085 |
| wwww | Factor 2, right hemisphere: female vehicle, female ACEA+GP1a | t(39.38) = 1.888, p = 0.0664 | Normal | t-test | –0.03766 to 1.099 |
| PN26 dual agonism masculinizes neuronal morphology | |||||
| xxxx | Factor 1, left hemisphere: male vehicle, female vehicle, female ACEA+GP1a | F(2, 72) = 0.257, p = 0.774 | Normal | ANOVA | 0.089 |
| yyyy | Factor 1, right hemisphere: male vehicle, female vehicle, female ACEA+GP1a | F(2, 72) = 5.689, p = 0.005 | Normal | ANOVA | 0.849 |
| zzzz | Factor 1, right hemisphere: male vehicle, female vehicle | p = 0.008 | Tukey's Post-hoc | –1.3958 to –0.1738 | |
| aaaaa | Factor 1, right hemisphere: female vehicle, female ACEA+GP1a | p = 0.012 | Tukey’s post hoc | 0.1473 to 1.4254 | |
| bbbbb | Factor 1, right hemisphere: male vehicle, female ACEA+GP1a | p = 1.000 | Tukey’s post hoc | –0.5597 to 0.5628 | |
| ccccc | Factor 2, left hemisphere: male vehicle, female vehicle, female ACEA+GP1a | F(2, 72) = 0.956, p = 0.775 | Normal | ANOVA | 0.089 |
| ddddd | Factor 2, right hemisphere: male vehicle, female vehicle, female ACEA+GP1a | F(2, 72) = 1.991, p = 0.144 | Normal | ANOVA | 0.398 |