a | Normal distribution | Welch’s t-test | PN5 hippocampus: VEH vs. LC | t((2.9847) = 5.667 | 0.011 | d = 4.625 | 0.984 |
b | Normal distribution | Welch's t-test | PN5 amygdala: VEH vs. LC | t(3.7237) = 5.996 | 0.005 | d = 2.38 | 0.596 |
c | Normal distribution | Welch's t-test | PN5 cortex: VEH vs. LC | t(3.3363) = 2.915 | 0.054 | d = 4.896 | 0.991 |
d | Normal distribution | Welch's t-test | PN5 hypothalamus: VEH vs. LC | t(3.7531) = 2.108 | 0.059 | d = 3.064 | 0.798 |
e | Normal distribution | Welch's t-test | PN10 hippocampus: VEH vs. LC | t(1.0548) = –0.875 | 0.536 | d = 1.045 | 0.13 |
f | Normal distribution | Welch's t-test | PN10 amygdala: VEH vs. LC | t(2.6755) = –3.941 | 0.036 | d = 2.461 | 0.457 |
g | Normal distribution | Welch's t-test | PN10 cortex: VEH vs. LC | t(1.0034) = –2.013 | 0.293 | d = 3.409 | 0.702 |
h | Normal distribution | Welch's t-test | PN10 hypothalamus: VEH vs. LC | t(1.196) = –1.257 | 0.401 | d = 1.42 | 0.197 |
i | Normal distribution | Welch's t-test | PN15 hippocampus: VEH vs. LC | t(1.0067) = –1.248 | 0.429 | d = 1.248 | 0.22 |
j | Normal distribution | Welch's t-test | PN15 amygdala: VEH vs. LC | t(1.1786) = –1.437 | 0.36 | d = 1.269 | 0.225 |
k | Normal distribution | Welch's t-test | PN15 cortex: VEH vs. LC | t(1.312) = –1.269 | 0.384 | d = 1.437 | 0.274 |
l | Normal distribution | Welch's t-test | PN15 hypothalamus: VEH vs. LC | t(1.0299) = 0.254 | 0.841 | d = 0.254 | 0.057 |
Body weight | | | | | | | |
m | Normal distribution | 3-way ANOVA | Main effect: sex | F(1,29) = 236.343 | <0.001 | η2 = 0.068 | 1 |
Female body weight | | | | | | | |
n | Normal distribution | 2-way ANOVA | Main effect: treatment | F(1,14) = 0.077 | 0.785 | η2 = 0.072 | 0.058 |
o | Normal distribution | 2-way ANOVA | Main effect: age | F(1.146,16.037) = 617.607 | <0.001 | η2 = 0.974 | 1 |
p | Normal distribution | 2-way ANOVA | Interaction: treatment × age | F(1.146,16.07) = 2.842 | 0.108 | η2 = 0.004 | 0.375 |
Male body weight | | | | | | | |
q | Normal distribution | 2-way ANOVA | Main effect: treatment | F(1,15) = 6.045 | 0.027 | η2 = 0.287 | 0.633 |
r | Normal distribution | 2-way ANOVA | Main effect: age | F(1.626,24.387) = 2222.172 | <0.001 | η2 = 0.992 | 1 |
s | Normal distribution | 2-way ANOVA | Interaction: treatment × age | F(1.626,24.387) = 2.019 | 0.161 | η2 = 0.001 | 0.341 |
Cliff aversion | | | | | | | |
t | Normal distribution | 3-way ANOVA | Main effect: sex | F(1,34) = 1.165 | 0.288 | η2 = 0.033 | 0.183 |
u | Normal distribution | 2-way ANOVA | Main effect: treatment | F(1,36) = 0.381 | 0.541 | η2 = 0.011 | 0.092 |
v | Normal distribution | 2-way ANOVA | Main effect: age | F(5,180) = 9.473 | <0.001 | η2 = 0.155 | 1 |
w | Normal distribution | 2-way ANOVA | Interaction: treatment × age | F(5,180) = 1.460 | 0.24 | η2 = 0.031 | 0.263 |
Surface righting | | | | | | | |
x | Normal distribution | 3-way ANOVA | Main effect: sex | F(1,34) = 0.177 | 0.676 | η2 = 0.004 | 0.069 |
y | Normal distribution | 2-way ANOVA | Main effect: treatment | F(1,36) = 9.999 | 0.003 | η2 = 0.217 | 0.868 |
z | Normal distribution | 2-way ANOVA | Main effect: age | F(5,180) = 3.973 | 0.014 | η2 = 0.094 | 0.944 |
aa | Normal distribution | 2-way ANOVA | Interaction: treatment × age | F(5,180) = 2.134 | 0.11 | η2 = 0.051 | 0.489 |
Wire hang | | | | | | | |
bb | Normal distribution | 3-way ANOVA | Main effect: sex | F(1,34) = 0.934 | 0.34 | η2 = 0.015 | 0.156 |
cc | Normal distribution | 2-way ANOVA | Main effect: treatment | F(1,36) = 25.742 | <0.001 | η2 = 0.083 | 0.999 |
dd | Normal distribution | 2-way ANOVA | Main effect: age | F(4,144) = 32.229 | <0.001 | η2 = 0.367 | 1 |
ee | Normal distribution | 2-way ANOVA | Interaction: treatment × age | F(4,144) = 2.223 | 0.087 | η2 = 0.032 | 0.561 |
Negative geotaxis | | | | | | | |
ff | Normal distribution | 3-way ANOVA | Main effect: sex | F(1,34) = 0.003 | 0.955 | η2 < 0.000 | 0.05 |
gg | Normal distribution | 2-way ANOVA | Main effect: treatment | F(1,36) = 12.68 | 0.001 | η2 = 0.024 | 0.934 |
hh | Normal distribution | 2-way ANOVA | Main effect: age | F(9,324) = 44.323 | <0.001 | η2 = 0.493 | 1 |
ii | Normal distribution | 2-way ANOVA | Interaction: treatment × age | F(9,324) = 1.228 | 0.291 | η2 = 0.015 | 0.492 |
Locomotion | | | | | | | |
jj | Normal distribution | 3-way ANOVA | Main effect: sex | F(1,34) = 1.109 | 0.3 | η2 = 0.008 | 0.176 |
kk | Normal distribution | 2-way ANOVA | Main effect: treatment | F(1,36) = 93.876 | <0.001 | η2 = 0.723 | 1 |
ll | Normal distribution | 2-way ANOVA | Main effect: age | F(9,324) = 26.712 | <0.001 | η2 = 0.378 | 1 |
mm | Normal distribution | 2-way ANOVA | Interaction: treatment × age | F(9,324) = 7.873 | <0.001 | η2 = 0.088 | 1 |
nn | Normal distribution | Welch's t-test | Post hoc: PN5 LC vs. VEH | t(29.15) = –0.398 | 0.694; α = 0.05 | d = 0.125 | 0.066 |
oo | Normal distribution | Welch's t-test | Post hoc: PN6 LC vs. VEH | t(34.126) = 0.766 | 0.449; α = 0.025 | d = 0.25 | 0.117 |
pp | Normal distribution | Welch's t-test | Post hoc: PN7 LC vs. VEH | t(24.031) = 1.869 | 0.074; α = 0.0125 | d = 0.628 | 0.469 |
qq | Normal distribution | Welch's t-test | Post hoc: PN8 LC vs. VEH | t(34.211) = 1.888 | 0.068; α = 0.01 | d = 0.618 | 0.456 |
rr | Normal distribution | Welch's t-test | Post hoc: PN9 LC vs. VEH | t(34.971) = 1.514 | 0.139; α = 0.01667 | d = 0.494 | 0.315 |
ss | Normal distribution | Welch’s t-test | Post hoc: PN10 LC vs. VEH | t(34.907) = 4.212 | <0.001; α = 0.00714 | d = 1.374 | 0.984 |
tt | Normal distribution | Welch’s t-test | Post hoc: PN11 LC vs. VEH | t(35.873) = 4.639 | <0.001; α = 0.0625 | d = 1.503 | 0.994 |
uu | Normal distribution | Welch’s t-test | Post hoc: PN12 LC vs. VEH | t(31.565) = 3.096 | 0.004; α = 0.0083 | d = 1.021 | 0.864 |
vv | Normal distribution | Welch’s t-test | Post hoc: PN13 LC vs. VEH | t(35.123) = 5.448 | <0.001; α = 0.0056 | d = 1.745 | 0.999 |
ww | Normal distribution | Welch’s t-test | Post hoc: PN14 LC vs. VEH | t(35.301) = 8.084 | <0.001; α = 0.005 | d = 2.631 | 1 |
Nest seeking total score | | | | | | | |
xx | Normal distribution | 2-way ANOVA | Main effect: sex | F(1,34) = 0.0002 | 0.989 | η2 < 0.000 | 0.05 |
yy | Normal distribution | Welch’s t-test | VEH vs. LC | t(34.052) = 3.927 | <0.001 | d = 1.285 | 0.97 |
Nest seeking over time | | | | | | | |
zz | Normal distribution | 3-way ANOVA | Main effect: sex | F(1,34) = 0.015 | 0.904 | η2 < 0.000 | 0.05 |
aaa | Normal distribution | 2-way ANOVA | Main effect: treatment | F(1,36) = 15.626 | <0.001 | η2 = 0.043 | 0.97 |
bbb | Normal distribution | 2-way ANOVA | Main effect: age | F(10,360) = 2.423 | 0.008 | η2 = 0.053 | 0.943 |
ccc | Normal distribution | 2-way ANOVA | Interaction: treatment × age | F(10,360) = 1.087 | 0.371 | η2 = 0.028 | 0.46 |
Nest seeking latency | | | | | | | |
ddd | Normal distribution | 3-way ANOVA | Main effect: sex | F(1,34) = 0.278 | 0.602 | η2 = 0.008 | 0.081 |
eee | Normal distribution | 2-way ANOVA | Main effect: age | F(10,360) = 47.962 | <0.001 | η2 = 0.458 | 1 |
fff | Normal distribution | 2-way ANOVA | Main effect: treatment | F(1,36) = 0.075 | 0.786 | η2 = 0.002 | 0.058 |
ggg | Normal distribution | 2-way ANOVA | Interaction: treatment × age | F(10,360) = 0.369 | 0.847 | η2 = 0.004 | 0.137 |
Isolation-induced USVs | | | | | | | |
hhh | Normal distribution | 2-way ANOVA | Main effect: sex | F(1,23) = 0.253 | 0.62 | η2 = 0.004 | 0.077 |
iii | Normal distribution | Welch’s t-test | VEH vs. LC | t(23.928) = 7.337 | <0.001 | d = 2.795 | 0.999 |
Spontaneous alternations | | | | | | | |
jjj | Normal distribution | 2-way ANOVA | Main effect: sex | F(1,34) = 1.802 | 0.188 | η2 = 0.043 | 0.257 |
kkk | Normal distribution | Welch’s t-test | VEH vs. LC | t(33.907) = 2.327 | 0.026 | d = 0.762 | 0.626 |
Social recognition index | | | | | | | |
lll | Normal distribution | 2-way ANOVA | Main effect: sex | F(1,25) = 0.299 | 0.59 | η2 = 0.01 | 0.062 |
mmm | Normal distribution | Welch’s t-test | VEH vs. LC | t(21.744) = 1.822 | 0.083 | d = 0.702 | 0.441 |
nnn | Normal distribution | One sample t-test | VEH vs. 0.50 | t(15) = 3.599 | 0.003 | d = 0.9 | 0.92 |
ooo | Normal distribution | One sample t-test | LC vs. 0.50 | t(12) = 0.224 | 0.827 | d = 0.062 | 0.055 |
Novel object: 1 h | | | | | | | |
ppp | Normal distribution | 2-way ANOVA | Main effect: sex | F(1,25) = 3.911 | 0.059 | η2 = 0.135 | 0.477 |
qqq | Normal distribution | Welch’s t-test | VEH vs. LC | t(21.868) = 0.119 | 0.907 | d = 0.046 | 0.052 |
Novel object: 24 h | | | | | | | |
rrr | Normal distribution | 2-way ANOVA | Main effect: sex | F(1,25) = 0.182 | 0.674 | η2 = 0.006 | 0.069 |
sss | Normal distribution | Welch’s t-test | VEH vs. LC | t(21.258) = –1.753 | 0.094 | d = 0.677 | 0.416 |
Open field center time | | | | | | | |
ttt | Normal distribution | 2-way ANOVA | Main effect: sex | F(1,34) = 0.061 | 0.806 | η2 = 0.002 | 0.057 |
uuu | Normal distribution | Welch’s t-test | VEH vs. LC | t(35.933) = –2.916 | 0.006 | d = 0.944 | 0.807 |
Open field grid crosses | | | | | | | |
vvv | Normal distribution | 2-way ANOVA | Main effect: sex | F(1,34) = 0.255 | 0.617 | η2 = 0.003 | 0.078 |
www | Normal distribution | Welch’s t-test | VEH vs. LC | t(25.858) = –7.427 | <0.001 | d = 2.484 | 1 |
Elevated plus maze % open time | | | | | | | |
xxx | Normal distribution | 2-way ANOVA | Main effect: sex | F(1,34) = 1.612 | 0.213 | η2 = 0.023 | 0.235 |
yyy | Normal distribution | Welch’s t-test | VEH vs. LC | t(31.406) = –5.85 | <0.001 | d = 1.929 | 0.999 |
PO time behind barrier | | | | | | | |
zzz | Normal distribution | 3-way ANOVA | Main effect: sex | F(1,34) = 0.0364 | 0.85 | η2 = 0.001 | 0.054 |
aaaa | Normal distribution | 2-way ANOVA | Main effect: treatment | F(1,36) = 10.208 | 0.003 | η2 = 0.158 | 0.875 |
bbbb | Normal distribution | 2-way ANOVA | Main effect: test phase | F(1,36) = 6.174 | 0.018 | η2 = 0.04 | 0.677 |
cccc | Normal distribution | 2-way ANOVA | Interaction: treatment × test phase | F(1,36) = 1.701 | 0.201 | η2 = 0.039 | 0.246 |
PO freezing duration | | | | | | | |
dddd | Normal distribution | 3-way ANOVA | Main effect: sex | F(1,34) = 0.002 | 0.967 | η2 < 0.000 | 0.05 |
eeee | Normal distribution | 2-way ANOVA | Main effect: treatment | F(1,36) = 3.616 | 0.065 | η2 = 0.091 | 0.457 |
ffff | Normal distribution | 2-way ANOVA | Main effect: test phase | F(1,36) = 0.589 | 0.448 | η2 = 0.016 | 0.116 |
gggg | Normal distribution | 2-way ANOVA | Interaction: treatment × test phase | F(1,36) = 0.955 | 0.335 | η2 = 0.025 | 0.158 |
PO stretch attend duration | | | | | | | |
hhhh | Normal distribution | 3-way ANOVA | Main effect: sex | F(1,34) = 0.0002 | 0.99 | η2 < 0.000 | 0.05 |
iiii | Normal distribution | 2-way ANOVA | Main effect: treatment | F(1,36) = 9.223 | 0.004 | η2 = 0.204 | 0.999 |
jjjj | Normal distribution | 2-way ANOVA | Main effect: test phase | F(1,36) = 27.268 | <0.001 | η2 = 0.389 | 0.999 |
kkkk | Normal distribution | 2-way ANOVA | Interaction: treatment × test phase | F(1,36) = 6.805 | 0.013 | η2 = 0.097 | 0.719 |
llll | Normal distribution | Welch’s t-test | Post hoc: VEH baseline vs. odor | t(19) = –4.95 | <0.001; α = 0.0125 | d = 1.459 | 0.994 |
mmmm | Normal distribution | Welch’s t-test | Post hoc: LC baselline vs. odor | t(17) = –2.249 | 0.038; α = 0.025 | d = 0.711 | 0.544 |
nnnn | Normal distribution | Welch’s t-test | Post hoc: baseline VEH vs. LC | t(29.935) = 1.313 | 0.199; α = 0.05 | d = 0.414 | 0.237 |
oooo | Normal distribution | Welch’s t-test | Post hoc: odor VEH vs. LC | t(34.037) = 3.026 | 0.005; α = 0.01667 | d = 0.965 | 0.824 |
PO stretch locomotion duration | | | | | | | |
pppp | Normal distribution | 3-way ANOVA | Main effect: sex | F(1,34) = 0.032 | 0.86 | η2 = 0.001 | 0.053 |
qqqq | Normal distribution | 2-way ANOVA | Main effect: treatment | F(1,36) = 11.985 | 0.001 | η2 = 0.25 | 0.92 |
rrrr | Normal distribution | 2-way ANOVA | Main effect: test phase | F(1,36) = 8.605 | 0.006 | η2 = 0.146 | 0.814 |
ssss | Normal distribution | 2-way ANOVA | Interaction: treatment × test phase | F(1,36) = 14.325 | <0.001 | η2 = 0.243 | 0.957 |
tttt | Normal distribution | Welch’s t-test | Post hoc: VEH baseline vs. odor | t(19) = 0.846 | 0.408; α = 0.05 | d = 0.209 | 0.099 |
uuuu | Normal distribution | Welch’s t-test | Post hoc: LC baselline vs. odor | t(17) = –3.755 | 0.002; α = 0.01667 | d = 0.739 | 0.577 |
vvvv | Normal distribution | Welch’s t-test | Post hoc: baseline VEH vs. LC | t(27.927) = –1.521 | 0.14; α = 0.025 | d = 0.506 | 0.329 |
wwww | Normal distribution | Welch’s t-test | Post hoc: odor VEH vs. LC | t(20.041) = –4.225 | <0.001; α = 0.0125 | d = 1.435 | 0.99 |
PO stimulus cloth approaches | | | | | | | |
xxxx | Normal distribution | 3-way ANOVA | Main effect: sex | F(1,34) = 0.032 | 0.86 | η2 = 0.001 | 0.053 |
yyyy | Normal distribution | 2-way ANOVA | Main effect: treatment | F(1,36) = 8.312 | 0.007 | η2 = 0.113 | 0.801 |
zzzz | Normal distribution | 2-way ANOVA | Main effect: test phase | F(1,36) = 19.22 | <0.001 | η2 = 0.135 | 0.989 |
aaaaa | Normal distribution | 2-way ANOVA | Interaction: treatment × test phase | F(1,36) = 1.116 | 0.298 | η2 = 0.02 | 0.177 |
PO stimulus cloth interaction duration | | | | | | | |
bbbbb | Normal distribution | 3-way ANOVA | Main effect: sex | F(1,34) = 0.067 | 0.798 | η2 = 0.001 | 0.057 |
ccccc | Normal distribution | 2-way ANOVA | Main effect: treatment | F(1,36) = 26.650 | <0.001 | η2 = 0.34 | 0.999 |
ddddd | Normal distribution | 2-way ANOVA | Main effect: test phase | F(1,36) = 0.521 | 0.475 | η2 = 0.014 | 0.108 |
eeeee | Normal distribution | 2-way ANOVA | Interaction: treatment × test phase | F(1,36) = 0.42 | 0.521 | η2 = 0.011 | 0.097 |
Male sex behavior | | | | | | | |
fffff | Non-normal | Wilcoxon rank sum test | Mount number: VEH vs. LC | W = 63 | 0.007 | HL = 18.889 | 5.999 to 35.999 |
ggggg | Non-normal | Wilcoxon rank sum test | Mount latency: VEH vs. LC | W = 8.5 | 0.011 | HL = -711.738 | -1180 to -25.999 |
hhhhh | Non-normal | Wilcoxon rank sum test | Intromission number: VEH vs. LC | W = 57.5 | 0.028 | HL = 9.0 | 4.33e-05 to 13.999 |
iiiii | Non-normal | Wilcoxon rank sum test | Intromission latency: VEH vs. LC | W = 8.5 | 0.009 | HL = -862.834 | -1167 to -121 |
jjjjj | Non-normal | Wilcoxon rank sum test | Ejaculation number: VEH vs. LC | W = 48.5 | 0.134 | HL < 0.000 | -6.933e-06 to 1 |
kkkkk | Non-normal | Wilcoxon rank sum test | Ejaculation latency: VEH vs. LC | W = 20 | 0.098 | HL = -131.621 | -663 to 5.161e-05 |
lllll | Normal distribution | Welch’s t-test | Hops and darts: VEH vs. LC | t(16.893) = 0.532 | 0.602 | d = 0.244 | 0.079 |
mmmmm | Normal distribution | Welch’s t-test | Solicitations: VEH vs. LC | t(16.501) = 1.307 | 0.209 | d = 0.602 | 0.236 |
nnnnn | Non-normal | Wilcoxon rank sum test | Lordosis quotient: VEH vs. LC | W = 45 | 1 | HL < 0.000 | -5.437e-06 to 2.74e-06 |
ooooo | Normal distribution | Welch’s t-test | Factor 1: male vs. female | t(26.023) = –0.5 | 0.621 | d = 0.187 | 0.077 |
ppppp | Normal distribution | Welch’s t-test | Factor 2: male vs. female | t(18.074) = –0.691 | 0.499 | d = 0.263 | 0.105 |
qqqqq | Normal distribution | Welch’s t-test | Factor 3: male vs. female | t(20.761) = 0.704 | 0.49 | d = 0.256 | 0.102 |
rrrrr | Normal distribution | Welch’s t-test | Factor 4: male vs. female | t(26.982) = –0.572 | 0.572 | d = 0.212 | 0.085 |
sssss | Normal distribution | Welch’s t-test | Factor 1: VEH vs. LC | t(26.941) = –3.267 | 0.003 | d = 1.187 | 0.865 |
ttttt | Normal distribution | Welch’s t-test | Factor 2: VEH vs. LC | t(25.663) = –4.063 | <0.001 | d = 1.449 | 0.962 |
uuuuu | Normal distribution | Welch’s t-test | Factor 3: VEH vs. LC | t(25.689) = 1.559 | 0.131 | d = 0.556 | 0.301 |
vvvvv | Normal distribution | Welch’s t-test | Factor 4: VEH vs. LC | t(21.724) = 1.415 | 0.171 | d = 0.545 | 0.291 |