Primers used in qRT-PCR and for generating in situ hybridization probes.
| Gene | Forward Primer | Reverse Primer | Amplicon Size (bp) |
| Used in RT-PCR | |||
| Zic2 | GCATGTCCACACCTCAGATAAG | ATGGACCTTCATGTGCTTCC | 324 |
| Slc6a4 (SERT) | GCTGAGATGAGGAACGAAGAC | GAGGAAGAAGATGATGGCAAAG | 1240 |
| Gja1 | TACCCAACAGCAGCAGACTTT | AAATGAAGAGCACCGACAGC | 240 |
| Klf4 | CCCAACTACCCTCCTTTCCT | AGGTTTCTCGCCTGTGTGAGT | 2175 |
| Lhx2 | GCGAATACCCAGCACACTTT | TGTTCAGCATCGTTCTCGTT | 235 |
| Otx2 | GTTCCGTCACTCCAAATCTACC | GTCCTCTCCCTTCGCTGTTT | 248 |
| Sox2 | GTTACCTCTTCCTCCCACTCCA | CTTCTCCAGTTCGCAGTCCA | 216 |
| Sparc | GACTCTTCCTGCCACTTCTTTG | AGGTTGTTGCCCTCATCTCTCT | 1454 |
| Zfp36 | CCCATCTTCAATCGTATCTCTG | CTGTCAACTGTCTCCCTCAAAC | 1765 |
| Zic1 | GCAAGATGTGCGATAAGTCC | GTGGTCGGGTTGTCTGTTGT | 744 |
| Sema3e | CCAACTCCTCCTTTGTGTCC | TCATCTCGGTCTTCGTTATCAG | 2570 |
| Tbx20 | ATTGAGAGGGAGAGTGTGGAGA | ACGATACCCAGGAACTGAGAGA | 1338 |
| Hprt | AGCAGGTGTTCTAGTCCTGTGG | ACGCAGCAACTGACATTTCTAA | 101 |
| Used for generating ISH probes | |||
| Ccnd2 | TCCTGTTTGCCTTCCTTGGAGCCT | ATATGACGGGCTCTGCTTTCCCGT | 566 |
| Fgf12 | GGAGACTTAGGGAACTCGCTGGCA | CACGACCCAAACCCACCCACAAAA | 500 |
| Igf1 | AGCAGATAGAGCCTGCGCAATGGA | CGGGGACTTCTGAGTCTTGGGCAT | 551 |
| Igfbp5 | CTCCAACCCGGAACATGGAGCAAC | GCCCACATAAGGGACAGAGGCTCA | 508 |
| Sox2 | ACAAGGGAATTGGGAGGGGTGCAA | AACCCAGCAAGAACCCTTTCCTCG | 504 |
| Tbx20 | TGGGTGAGCTGACGAGTCTGGATG | TATGATGTGCACCCGTGGCTGGTA | 536 |
| Zic2 | AACCCATCGAGGGCACCTTAGGAT | AGGGAGACTTTGGCACGGCTCATA | 600 |