CIP step | Signature feature | Characteristic measurements of feature | Selected IS3 cell measurement values | Elimination criteria (step 2) |
---|---|---|---|---|
−100 pA | Lack of “sag” | (1) Hyperpolarization Vm Difference (mean pulse value − mean initial value) | −34.2649 mV | None |
(2) Minimum potential time | 58.9000 ms | |||
(3) Minimum potential | −112.6709 mV | |||
(4) Potential sag | 6.7865 mV | |||
5) Sag time constant | 14.3000 ms | |||
20 pA | No spiking | (1) Passive depolarization Vm difference (mean pulse value − mean initial value) | 12.4910 mV | If spikes are observed |
50 pA | Normal spiking | (1) Active depolarization Vm difference (mean pulse value − mean initial value) | 29.4174 mV | If fewer than three spikes are observed If membrane potential fails to repolarize |
(2) Interspike interval | 36.6898 ms | |||
(3) First spike time | 33.1000 ms | |||
(4) Spike voltage threshold mean | −44.3428 mV | |||
(5) Spike half-width mean | 1.0301 ms | |||
(6) Spike amplitude mean | 61.7296 mV | |||
(7) Spike rate | 35.0044 Hz | |||
8) Spike maximum afterhyperpolarization mean | 6.8820 mV | |||
(9) Number of spikes | 28.0000 | |||
(10) Spike frequency adaptation | 1.3305 | |||
500 pA | Depolarization block | (1) Depolarization block Vm difference (final 700 ms mean pulse value − mean initial value) | 62.6461 mV | If spikes in the last 700 ms of the CIP are observed If membrane potential fails to repolarize If spikes in the recovery period are observed |
(2) Initial 100 ms spike rate | 60.0000 Hz |
For each CIP step (1st column) we note a signature feature that is observed experimentally (2nd column), characteristic measurements that are used to quantify the signature feature (3rd column), characteristic measurement values from the selected IS3 traces (4th column), as well as conditions where models would be rejected for clearly not capturing the experimental feature (5th column). CIP, Current Injection Protocol; Vm, membrane potential.