Primer sequences used in qPCR
| Gene | Forward primer 5'-3' | Reverse primer 5'-3' | Tm |
|---|---|---|---|
| Vegf | CCA GGC TGC ACC CAC GAC AG | TGA GGT GTG GGG GCT GCT GT | F: 63.5R: 64.1 |
| Hif1α | CTG TGA TGA AAG AAT TAC TGA GTT GAT G | CAT AAA TTG AGC GGC CCA AA | F: 54.3R: 53.9 |
| Tgfβr3 | CAG GAC CAG CTC GAT GGA A | CAC CAG GAA GAG GTC TGT TGT TAT ACA | F: 56.9R: 58.0 |
| Timp1 | CAG TCC CTG CCG CCA TCG TC | TGT GGG TGG AGT GGG GCA CA | F: 63.2R: 64.1 |
| Clic1 | TGG ACC GAG CGG AGG GTC TG | CCA TGG TTG CGT CGG GGA CC | F: 63.8R: 63.6 |
| Mmp2 | CAC AAG TGG CCT GGG GAG CG | GCG TGG CTT CCG CAT GGT CT | F: 63.6R: 63.2 |
| Csf2 | CTG CTC CCA CTC GCT CAC CC | AGG TTG CCC CGT AGG CCC TT | F: 62.8R: 64.0 |
| 18s | GTA ACC CGT TGA ACC CCA TT | CCA TCC AAT CGG TAG TAG CG | F: 55.3R: 55.1 |
| Vwf | CCG GAA GCG ACC CTC AGA | CGG TCA ATT TTG CCA AAG ATC T | F: 59.5R: 54.0 |
| Gfap | CAC GTG GAG ATG GAT GTG GC | CAG TTG GCG GCG ATA GTC ATT A | F: 58.4R: 57.2 |
| Nfh | CGA GCT GTA CGA GCG CGA GG | AGC TCG CCC ACC TCC TCC TG | F: 62.8R: 64.1 |
F, Forward; R, reverse.