Experiment 2 statistical analyses
| Data structure | Dependent variable | Comparison | Type of test | p value | Power | |
|---|---|---|---|---|---|---|
| d1 | Normal distribution | Total play | Main effect of age | Three-way ANOVA | <0.0001 | 0.997 |
| d2 | Normal distribution | Main effect of genotype | Three-way ANOVA | <0.0001 | 1.000 | |
| d3 | Normal distribution | Main effect of sex | Three-way ANOVA | 0.6259 | 0.076 | |
| d4 | Normal distribution | Hom vs WT | Fisher's PLSD | <0.0001 | 1.000 | |
| d5 | Normal distribution | Hom vs Het | Fisher's PLSD | <0.0001 | 1.000 | |
| e1 | Non-normal distribution | Pins | Main effect of age | Three-way ANOVA, M-W | 0.0041 (ANOVA), 0.0002 (M-W) | 0.843 |
| e2 | Non-normal distribution | Main effect of genotype | Three-way ANOVA, K-W | 0.0031 (ANOVA), <0.0001 (K-W) | 0.890 | |
| e3 | Non-normal distribution | Main effect of sex | Three-way ANOVA, M-W | 0.9169 (ANOVA), 0.3571 (M-W) | 0.051 | |
| e4 | Non-normal distribution | Hom vs WT | Fisher's PLSD, M-W | 0.0048 (Fisher's), <0.0001 (M-W) | 0.908 | |
| e5 | Non-normal distribution | Hom vs Het | Fisher's PLSD, M-W | 0.0016 (Fisher's), <0.0001 (M-W) | 0.903 | |
| e6 | Non-normal distribution | genotype x sex, P44 | Two-way ANOVA | 0.0076 | 0.826 | |
| e7 | Normal distribution | Het male vs Het female, P44 | Fisher's PLSD | 0.0019 | 0.921 | |
| f1 | Normal distribution | Play attacks | Main effect of age | Three-way ANOVA | <0.0001 | 0.999 |
| f2 | Normal distribution | Main effect of genotype | Three-way ANOVA | <0.0001 | 1.000 | |
| f3 | Normal distribution | Main effect of sex | Three-way ANOVA | 0.5730 | 0.085 | |
| f4 | Normal distribution | Hom vs WT | Fisher's PLSD | <0.0001 | 1.000 | |
| f5 | Normal distribution | Hom vs Het | Fisher's PLSD | <0.0001 | 1.000 | |
| g1 | Normal distribution | Total social behaviors | Main effect of age | Three-way ANOVA | <0.0001 | 1.000 |
| g2 | Normal distribution | Main effect of genotype | Three-way ANOVA | <0.0001 | 1.000 | |
| g3 | Normal distribution | Main effect of sex | Three-way ANOVA | 0.8892 | 0.052 | |
| g4 | Normal distribution | Hom vs WT | Fisher's PLSD | <0.0001 | 1.000 | |
| g5 | Normal distribution | Hom vs Het | Fisher's PLSD | <0.0001 | 1.000 | |
| h1 | Normal distribution | Social Investigation/allogrooming | Main effect of age | Three-way ANOVA | 0.0090 | 0.758 |
| h2 | Normal distribution | Main effect of genotype | Three-way ANOVA | 0.5137 | 0.156 | |
| h3 | Normal distribution | Main effect of sex | Three-way ANOVA | 0.4631 | 0.109 | |
| i1 | Non-normal distribution | Huddling | Main effect of age | Three-way ANOVA, M-W | <0.0001 (ANOVA), 0.0001 (M-W) | 0.999 |
| i2 | Non-normal distribution | Main effect of genotype | Three-way ANOVA, K-W | <0.0001 (ANOVA), <0.0001 (K-W) | 1.000 | |
| i3 | Non-normal distribution | Main effect of sex | Three-way ANOVA, M-W | 0.8084 (ANOVA), 0.8321 (M-W) | 0.057 | |
| i4 | Normal distribution | Hom vs WT | Fisher's PLSD, M-W | <0.0001 (Fisher's), 0.0003 (M-W) | 0.948 | |
| i5 | Non-normal distribution | Hom vs Het | Fisher's PLSD, M-W | <0.0001 (Fisher's), <0.0001 (M-W) | 1.000 | |
| j1 | Normal distribution | All USVs | Main effect of genotype | Three-way ANOVA | <0.0001 | 0.993 |
| j2 | Normal distribution | Hom vs WT | Fisher's PLSD | 0.0020 | 0.942 | |
| j3 | Normal distribution | Hom vs Het | Fisher's PLSD | <0.0001 | 0.998 | |
| k1 | Normal distribution | 50 kHz USVs | Main effect of age | Three-way ANOVA | 0.0380 | 0.537 |
| k2 | Normal distribution | Main effect of genotype | Three-way ANOVA | 0.0001 | 0.990 | |
| k3 | Normal distribution | Hom vs WT | Fisher's PLSD | 0.0022 | 0.947 | |
| k4 | Normal distribution | Hom vs Het | Fisher's PLSD | <0.0001 | 0.996 | |
| l1 | Non-normal distribution | 22 kHz USVs | Main effect of age | Three-way ANOVA, M-W | 0.0001 (ANOVA), <0.0001 (M-W) | 0.986 |
| l2 | Non-normal distribution | Main effect of genotype | Three-way ANOVA, K-W | 0.2541 (ANOVA), 0.2262 (K-W) | 0.282 |
K-W, Kruskal–Wallis test; M-W, Mann–Whitney U test.