Sequences of primer pairs and probes used for real-time RT-PCR
| Gene GenBack accession no. | Sequence 5′–3′ | Fragment size, bp | |
|---|---|---|---|
| Bdnf | (X55573) | 110 | |
| Forward primer | TGCAGGGGCATAGACAAAAGG | ||
| Reverse primer | CTTATGAATCGCCAGCCAATTCTC | ||
| Taqman Probe | ACTGGAACTCGCAATGCCGAACTACCCA | ||
| Ntf4 | (NM[lowem]198190) | 95 | |
| Forward primer | AGCGTTGCCTAGGAATACAGC | ||
| Reverse primer | GGTCATGTTGGATGGGAGGTATC | ||
| Taqman Probe | TGAGCAGTGAACCCGACCACCCAGG | ||
| Ntf3 | (NM-001164034) | ||
| Forward primer | CAGAACATAAGAGTCACCGGAA | 94 | |
| Reverse primer | TGTCCCCGAATGTCAATGG | ||
| Taqman Probe | CACCCACAGGCTCTCACTGTC | ||
| TrkB | (X17647) | 86 | |
| Forward primer | AAGGACTTTCATCGGGAAGCTG | ||
| Reverse primer | TCGCCCTCCACACAGACAC | ||
| Taqman Probe | CCAACCTCCAGCACGAGCACATTGTCAA | ||
| P2rx3 | (MN-145526) | 113 | |
| Forward primer | TTTCCCCTGGCTACAACTTC | ||
| Reverse primer | CCCGTATACCAGCACATCAAAG | ||
| Taqman Probe | AGA TGG AGA ATG GCA GCG AGT ACC G | ||
| 18S rRNA | (X00686) | CAGGATTGACAGATTGATAGCTCTTTC | 76 |
| Forward primer | ATCGCTCCACCAACTAAGAACG | ||
| Reverse primer | CCATGCACCACCACCCACGGAATCG | ||
| Taqman Probe | 130 | ||
| GAPDH | (NM_008084) | AATGTGTCCGTCGTGGATCTG | |
| Forward primer | CAACCTGGTCCTCAGTGTAGC | ||
| Reverse primer | CGTGCCGCCTGGAGAAACCTGCC | ||
| Taqman Probe | |||
| β-Actin | (NM_007393) | CTGGGACGACATGGAGAAGATC | 144 |
| Forward primer | GTCTCAAACATGATCTGGGTCATC | ||
| Reverse primer | ACCTTCTACAATGAGCTGCGTGTGGCC | ||
| Taqman Probe | |||