Figure | Description | Data structure | Type of test | p value | |
---|---|---|---|---|---|
A | 3A | Comparison between stimulations at cold (4°C), cool (21°C) neutral (25°C), hot (34°C) | Paired, non-parametric | Kruskal–Wallis ANOVA | <0.0001 |
Cold vs cool | Dunn’s post hoc test | n.s. | |||
Cold vs neutral | Dunn’s post hoc test | <0.0001 | |||
Cold vs hot | Dunn’s post hoc test | <0.0001 | |||
Cool vs neutral | Dunn’s post hoc test | <0.01 | |||
Cool vs hot | Dunn’s post hoc test | <0.01 | |||
Neutral vs hot | Dunn’s post hoc test | n.s. | |||
B | 3B | Comparison between cold stimulations (4°C), cold -5N, and cold -obex | Paired, non-parametric | Kruskal–Wallis ANOVA | 0.0411 |
Cold vs cold -5N | Dunn’s post hoc test | n.s. | |||
Cold vs cold -obex | Dunn’s post hoc test | < 0.05. | |||
Cold -5N vs cold -obex | Dunn’s post hoc test | n.s. | |||
C | 4A | Comparison between stimulations at cold (4°C), neutral (22°C), hot (45°C), and cold -obex | Paired, non-parametric | Friedman ANOVA | <0.0001 |
Cold vs neutral | Dunn’s post hoc test | <0.001 | |||
Cold vs hot | Dunn’s post hoc test | <0.001 | |||
Cold vs cold -obex | Dunn’s post hoc test | n.s. | |||
Neutral vs hot | Dunn’s post hoc test | n.s. | |||
Neutral vs cold -obex | Dunn’s post hoc test | <0.05 | |||
Hot vs cold -obex | Dunn’s post hoc test | n.s. | |||
D | N/A | Comparison between responses in Figures 3, 4A when different temperatures are used | Non-parametric | ||
Neutral 22°C vs neutral 25°C | Kolmogorov–Smirnov t test | 0.2644 | |||
Hot 34°C vs 45°C | Kolmogorov–Smirnov t test | 0.0495 | |||
-obex with bath at 25°C vs 22°C | Kolmogorov–Smirnov t test | <0.01 | |||
E | N/A | Comparison of response rates to cold (4°C) and neutral (22°C) following anesthesia by hypothermia or isoflurane | Non-parametric | ||
Cold hypothermia vs isoflurane | Kolmogorov–Smirnov t test | 0.3077 | |||
Neutral hypothermia vs isoflurane | Kolmogorov–Smirnov t test | 0.3874 | |||
F | 4B | Comparison between stimulations at cold (4°C), neutral (22°C), hot (45°C), cold -skin, neutral -skin, hot -skin, and cold -obex | Paired, non-parametric | Friedman ANOVA | <0.0001 |
Cold vs neutral | Dunn’s post hoc test | <0.01 | |||
Cold vs hot | Dunn’s post hoc test | <0.01 | |||
Cold vs cold -skin | Dunn’s post hoc test | n.s. | |||
Cold vs neutral -skin | Dunn’s post hoc test | <0.0001 | |||
Cold vs hot -skin | Dunn’s post hoc test | <0.0001 | |||
Cold vs cold -obex | Dunn’s post hoc test | <0.001 | |||
Neutral vs hot | Dunn’s post hoc test | n.s. | |||
Neutral vs cold -skin | Dunn’s post hoc test | <0.05 | |||
Neutral vs neutral -skin | Dunn’s post hoc test | n.s. | |||
Neutral vs hot -skin | Dunn’s post hoc test | n.s. | |||
Neutral vs cold -obex | Dunn’s post hoc test | n.s. | |||
Hot vs cold -skin | Dunn’s post hoc test | <0.05 | |||
Hot vs neutral -skin | Dunn’s post hoc test | n.s. | |||
Hot vs hot -skin | Dunn’s post hoc test | n.s. | |||
Hot vs cold -obex | Dunn’s post hoc test | n.s. | |||
Cold -skin vs neutral -skin | Dunn’s post hoc test | <0.0001 | |||
Cold -skin vs hot -skin | Dunn’s post hoc test | <0.001 | |||
Cold -skin vs cold -obex | Dunn’s post hoc test | <0.01 | |||
Neutral -skin vs hot -skin | Dunn’s post hoc test | n.s. | |||
Neutral -skin vs cold -obex | Dunn’s post hoc test | n.s. | |||
Hot -skin vs cold -obex | Dunn’s post hoc test | n.s. | |||
Cold vs cold-skin | Wilcoxon t test | 0.25 | |||
Cold vs cold -obex | Wilcoxon t test | 0.0010 | |||
Hot vs hot -skin | Wilcoxon t test | 0.0898 | |||
Neutral vs neutral -skin | Wilcoxon t test | 0.0078 | |||
Cold -skin vs cold-obex | Wilcoxon t test | 0.0015 | |||
G | 6A | EMG amplitudes for cold (4°C), neutral (22°C), hot (45°C) cold -5N, neutral -5N, hot -5N, and cold -obex | Unpaired, non-parametric | Kruskal–Wallis ANOVA | <0.0001 |
Cold vs neutral | Dunn’s post hoc test | <0.0001 | |||
Cold vs hot | Dunn’s post hoc test | <0.0001 | |||
Cold vs cold -5N | Dunn’s post hoc test | <0.0001 | |||
Cold vs neutral -5N | Dunn’s post hoc test | <0.0001 | |||
Cold vs hot-5N | Dunn’s post hoc test | <0.0001 | |||
Cold vs cold -obex | Dunn’s post hoc test | <0.0001 | |||
Neutral vs hot | Dunn’s post hoc test | n.s | |||
Neutral vs cold -5N | Dunn’s post hoc test | <0.0001 | |||
Neutral vs neutral -5N | Dunn’s post hoc test | <0.01 | |||
Neutral vs hot -5N | Dunn’s post hoc test | n.s. | |||
Neutral vs cold -obex | Dunn’s post hoc test | n.s. | |||
Hot vs cold -5N | Dunn’s post hoc test | <0.001 | |||
Hot vs neutral -5N | Dunn’s post hoc test | <0.01 | |||
Hot vs hot -5N | Dunn’s post hoc test | n.s. | |||
Hot vs cold -obex | Dunn’s post hoc test | n.s. | |||
Cold -5N vs neutral -5N | Dunn’s post hoc test | <0.0001 | |||
Cold -5N vs hot-5N | Dunn’s post hoc test | <0.0001 | |||
Cold -5N vs cold -obex | Dunn’s post hoc test | <0.001 | |||
Neutral -5N vs hot -5N | Dunn’s post hoc test | n.s. | |||
Neutral -5N vs cold -obex | Dunn’s post hoc test | <0.05 | |||
Hot -5N vs cold -obex | Dunn’s post hoc test | n.s. | |||
H | 6B | EMG amplitudes comparisons for cold (4°C), neutral (22°C), hot (45°C) when all responses are considered and when only responses >0 are considered | |||
Cold vs cold >0 | Kolmogorov–Smirnov t test | 0.9998 | |||
Neutral vs neutral >0 | Kolmogorov–Smirnov t test | <0.0001 | |||
Hot vs hot >0 | Kolmogorov–Smirnov t test | <0.0001. | |||
I | 7 | Comparisons of latencies for cold (4°C), neutral (22°C), and hot (45°C) | Unpaired, non-parametric | Kruskal–Wallis ANOVA | <0.0001 |
Cold vs neutral | Dunn’s post hoc test | <0.0001 | |||
Cold vs hot | Dunn’s post hoc test | <0.0001 | |||
Neutral vs hot | Dunn’s post hoc test | <0.0001 | |||
J | N/A | Comparison of amplitudes after cold (4°C) stimulations for right and left FL | Unpaired, non-parametric | Kolmogorov–Smirnov t test | 0.1726 |
K | N/A | Comparison of latencies after cold (4°C) stimulations for right and left FL | Unpaired, non-parametric | Kolmogorov–Smirnov t test | 0.6001 |
-5N, trigeminal transection; -obex, complete transection of the spinoencephalic junction, caudal to the obex; -skin, facial skin removal; N/A, non-applicable; n.s., not significant.