Table 1.

Mean ± SEM and number of observations for each separate set of out data (T and E2 birds), and results of the two-way GLM mixed-effect analysis of these data (F and associated probabilities)

VariableT outE2 outT vs E2DaysInteraction
Mean ± SEM (n)Mean ± SEM (n)F, pF, pF, p
Number of songs D727.7 ± 17.6 (4)122 ± 57 (5)
D1437.5 ± 30.8 (4)83.8 ± 38.9 (5)
D2831.7 ± 12.3 (4)17.0 ± 11.9 (5)F = 1.204,
p = 0.308
F = 1.783,
p = 0.216
F = 1.961,
p = 0.177
Song duration D70.34 ± 0.11 (4)0.28 ± 0.12 (5)
D140.35 ± 1.12 (4)0.39 ± 0.10 (5)
D280.44 ± 0.02 (4)0.28 ± 0.11 (5)F = 0.245,
p = 0.629
F = 0.303,
p = 0.674
F = 0.809,
p = 0.465
% Time singing D70.41 ± 0.22 (4)1.61 ± 0.77 (5)
D140.53 ± 0.38 (4)1.13 ± 0.52 (5)
D280.40 ± 0.17 (4)0.22 ± 0.15 (5)F = 1.123,
p = 0.324
F = 2.013,
p = 0.187
F = 1.792,
p = 0.203
Maximum frequency D73553 ± 328 (3)4095 ± 239 (3)
D143580 ± 342 (3)3727 ± 439 (4)
D283972 ± 403 (4)4176 ± 213 (3)F = 0.189,
p = 0.678
F = 0.682,
p = 0.484
F = 0.519,
p = 0.614
Bandwidth D71104 ± 167 (3)1885 ± 834 (3)
D141128 ± 97(3)2156 ± 1185 (4)
D28947 ± 141 (4)1268 ± 329 (3)F = 1.036,
p = 0.348
F = 0.499,
p = 0.612
F = 0.349,
p = 0.715
Mean entropy D72.98 ± 0.21 (3)3.29 ± 0.39 (3)
D142.85 ± 0.24 (3)2.93 ± 0.15 (4)
D282.74 ± 0.16 (4)2.92 ± 0.10 (3)F = 0.524,
p = 0.496
F = 4.037,
p = 0.128
F = 1.875,
p = 0.245
VariableT outE2 outT vs E2ipsilateral-contralateralInteraction
POM volume ipsilateral
contralateral
0.06 ± 0.01 (4)
0.05 ± 0.01 (4)
0.02 ± 0.02 (3)
0.02 ± 0.01 (3)
F = 3.022, p = 0.142F = 0.811,
p = 0.408
F = 0.227,
p = 0.653
HVC volume Nissl ipsilateral
contralateral
0.10 ± 0.02 (4)
0.11 ± 0.02 (4)
0.14 ± 0.02 (4)
0.12 ± 0.02 (4)
F = 0.810, p = 0.403F = 0.516,
p = 0.499
F = 1.507,
p = 0.265
HVC volume DCX ipsilateral
contralateral
0.11 ± 0.03 (4)
0.10 ± 0.02 (4)
0.10 ± 0.02 (5)
0.10 ± 0.01 (5)
F = 0.053, p = 0.824F = 1.032,
p = 0.343
F = 0.002,
p = 0.967
Fusiform DCX in HVC ipsilateral
contralateral
86.2 ± 12.3 (4)
64.5 ± 10.7 (4)
56.0 ± 6.9 (5)
53.6 ± 4.6 (5)
F = 3.559, p = 0.101F = 5.143,
p = 0.058
F = 3.302,
p = 0.112
Multipolar DCX in HVC ipsilateral
contralateral
152.0 ± 35.2 (4)
142.0 ± 30.7 (4)
151.4 ± 9.7 (5)
144.4 ± 8.0 (5)
F = 0.001, p = 0.976F = 1.715
p = 0.232
F = 0.053,
p = 0.824
Fusiform DCX vtr. HVC ipsilateral
contralateral
11.2 ± 3.1 (4)
10.0 ± 2.6 (4)
7.8 ± 0.8 (5)
7.8 ± 2.6 (5)
F = 1.099, p = 0.329F = 0.110,
p = 0.750
F = 0.110,
p = 0.750
Multipolar DCX vtr. HVC ipsilateral
contralateral
19.0 ± 3.8 (4)
18.5 ± 2.6 (4)
23.0 ± 2.8 (5)
21.4 ± 2.1 (5)
F = 1.255, p = 0.300F = 0.161,
p = 0.700
F = 0.044,
p = 0.839
Fusiform DCX lat. HVC ipsilateral
contralateral
13.0 ± 3.5 (4)
13.2 ± 2.5 (4)
7.4 ± 0.7 (5)
10.8 ± 2.4 (5)
F = 1.848, p = 0.216F = 1.401,
p = 0.275
F = 1.043,
p = 0.341
Multipolar DCX lat. HVC ipsilateral
contralateral
8.0 ± 1.3 (4)
6.5 ± 1.0 (4)
7.8 ± 1.5 (5)
8.6 ± 1.3 (5)
F = 0.369, p = 0.562F = 0.123,
p = 0.736
F = 1.332,
p = 0.286
PNN density ipsilateral
contralateral
36.7 ± 7.0 (3)
58.0 ± 22.1(3)
34.8 ± 20.4 (5)
46.4 ± 22.6 (5)
F = 0.070, p = 0.800F = 0.835,
p = 0.396
F = 0.072,
p = 0.796
PV-ir density ipsilateral
contralateral
106.3 ± 17.1 (3)
104.0 ± 0.0 (3)
116.6 ± 17.6 (5)
143.0 ± 25.7 (5)
F = 1.289, p = 0.278F = 0.308,
p = 0.589
F = 0.439,
p = 0.520
PV+PNN density ipsilateral
contralateral
6.0 ± 3.5 (3)
11.3 ± 3.9 (3)
7.6 ± 6.4 (5)
10.4 ± 4.6 (5)
F = 0.003, p = 0.957F = 0.618,
p = 0.462
F = 0.059,
p = 0.815
% PNN with PV ipsilateral
contralateral
21.3 ± 14.9 (3)
22.7 ± 5.4 (3)
2.2 ± 2.2 (5)
44.4 ± 23.1 (5)
F = 0.006, p = 0.938F = 1.744,
p = 0.213
F = 1.537,
p = 0.238