Colocalization of ANG II-induced Fos expression with CaMKIIa neurons in the MnPO
| Treatment | Total cFos+ | Total CaMKIIa+ | Total DL cells | % DL cFos+ |
|---|---|---|---|---|
| Control CNO + 0.9% SAL | 21.0 ± 10.0 | 182.8 ± 54.8 | 8.8 ± 1.25 | 50.2 ± 17.9% |
| Control VEH + 0.9% SAL | 25.8 ± 7.8 | 181.5 ± 37.5 | 15.1 ± 3.4 | 60.0 ± 4.8% |
| Control CNO + ANG II | 79.8 ± 8.7** | 195.0 ± 57.6 | 44.0 ± 7.8** | 54.4 ± 6.7% |
| Gi DREADD CNO + ANG II | 41.9 ± 7.2 | 206.5 ± 41.0 | 2.9 ± 1.0 | 6.5 ± 2.9%** |
ANG II exposure significantly increases Fos expression in the MnPO, specifically in CaMKIIa-positive neurons. CNO-mediated inhibition significantly attenuated ANG II-induced Fos expression in the MnPO in Gi DREADD-injected rats, with inhibition of the CaMKIIa neuronal phenotype; Bold text and **p < 0.001 compared to all groups. Data are presented as mean ± SEM.