Table 1.

Drd1a and Drd2 caudate-putamen medium spiny neuron properties compared across studies

PropertyKreitzer and Malenka, 2007aGertler et al., 2008Cepeda et al., 2008Ade et al., 2008cChan et al., 2012Planert et al., 2013Planert et al., 2013Goodliffe et al., 2018Current study, 2019
AnimalMiceMiceMiceMiceMiceMiceRatsMiceMice
MSN subtype identificationM4- or D2-eGFP BAC transgenic miceD1 and D2 receptor-eGFP BAC transgenic
mice on an FVB background		D1 and D2 receptor-eGFP BAC transgenic miceD1 and D2 receptor-eGFP BAC transgenic mice on a C57BL/6J backgroundD1 and D2 receptor-eGFP BAC transgenic mice on either a FVB/NJ or C57BL/6J backgroundD1 receptor-eGFP BAC transgenic miceRetrograde labeling of striatonigral MSNsD1 and D2 receptor-eGFP BAC transgenic mice on a C57BL/6J backgroundB6 Cg-Tg (Drd1a-tdTomato) 6 Calak/J hemizygous mice ona C57BL/6J background
Animal ageP20–P25P17–P70P39.7 + 1.6P16–P25P21–P35P15, P21–P32P14–P19∼P365P17–P22
Animal sexNot reportedNot reportedNot reportedMale and female data pooled regardless of sexMaleNot reportedNot reportedMale and Female data pooled regardless of sexMale and Female data analyzed by sex
Resting membrane potentialD1 = D2D1<D2—D1 = D2—D1 = D2D1 = D2D1 = D2D1 = D2
Rheobase—D1>D2———D1>D2D1>D2D1>D2D1>D2
AP threshold—D1 = D2D1>D2——D1 = D2D1 = D2D1>D2D1>D2
AP amplitude——D1 = D2————D1 = D2D1 = D2
AP amplitude change from first to second AP—————D1 = D2D1>D2——
AP width——D1 = D2——D1 = D2D1 = D2—D1>D2
AHP peak——D1 = D2—————D1 = D2
AHP time to Peak————————D1 = D2
Frequency of evoked action potentials/FI slopeD1<D2D1<D2—D1<D2D1<D2D1 = D2D1 = D2—D1<D2
Linear range input resistanceD1 = D2D1<D2D1 = D2D1 = D2—D1 = D2D1<D2D1 = D2D1<D2
Rectified range input resistance—————D1 = D2D1 = D2—D1 = D2
Inward rectification, %————————D1 = D2
Time constant of the membrane—D1>D2D1 = D2——D1 = D2D1<D2D1<D2D1 = D2
Capacitance—D1>D2D1 = D2—————D1 = D2
sEPSC frequency——D1<D2——————
sEPSC amplitude——D1 = D2——————
sEPSC kinetics——D1 = D2——————
mEPSC frequencyD1<D2—D1<D2————D1 = D2D1 = D2
mEPSC amplitudeD1 = D2—D1 = D2————D1 = D2D1<D2
mEPSC decayD1 = D2—D1 = D2—————D1 = D2
mEPSC rise time——D1<D2—————D1 = D2
s/mIPSC frequency———D1 = D2—————
s/mIPSC amplitude———D1 = D2—————
s/mIPSC decay———D1 = D2—————
s/mIPSC rise time———D1 = D2—————
Probability of occurrence of spontaneous membrane depolarization after GABAA blockade——D1<D2——————
Paired-pulse ratioD1>D2—D1<D2——————
AMPA-induced current amplitude——D1>D2——————
NMDA/AMPA ratioD1<D2————————
Endocannabinoid-mediated LTDD1<D2————————
Tonic GABAA current and sensitivity to GABAA current——D1<D2D1<D2—————

  • Only caudate-putamen MSN subtype electrophysiology studies in acute brain slice preparation experiments independent of variables such as dopamine depletion and psychostimulant exposure are included. This criteria a priori excludes studies that analyzed MSN subtype electrophysiological properties but did not directly compare D1 and D2 subtype groups (Day et al., 2006; Ade et al., 2011), or were performed in regions such as the nucleus accumbens (Ma et al., 2012; Cao et al., 2018). —, Did not measure; AP, action potential; AHP, afterhyperpolarization; FI, Frequency of evoked spikes to injected depolarization current; LTD, long-term depression.

  • aThe use of M4 eGFP labeling as equivalent to the D1 MSN subtype has been cautioned (Cepeda et al., 2008).

  • bThis finding significant in some but not all analyses within this study.

  • cA number of studies from Vicini and colleagues have investigated GABA conductance between MSN subtypes; here we feature the initial report.

  • dPlanert et al. (2013) assessed rheobase using multiple analyses. The conclusion of all analyses was similar and is thus condensed here.