Number of Lhx1+5/Evx2/Maf/Esrrg quadruple-positive neurons at different developmental stages in the mouse
| Stage | r5-cMVST lateral | Medial extension | N preparations | Sections assessed/preparation |
|---|---|---|---|---|
| E9.5 | 0 | 0 | 3 | Every 4th |
| E11.5 | 8 | 2 | 1 | Every 4th |
| E13.5a | 85 ± 25 | 25 ± 21 | 3 | Every 6th |
| E15.5b | 98 ± 21 | 25 ± 9 | 3 | Every 6th |
Counts of quadruple positive cells ± SD in different areas of the mouse CNS.
aCounts represent entire CNS. Only the hindbrain was assessed at other stages.
bPreparations at E15.5 were not coimmunostained for Lhx1+5 because they included retrograde labeling of the cMVST; here, counts represent Evx2/Maf/Esrrg immunostained neurons in and around the cMVST domain only (other brainstem areas were not assessed; Fig. 9).