Table 1.

Summary of all injections

StrainnInjected withInjection siteInjection amountFigure
C57BL/6J4 (3 M, 1 F)RetrobeadsOB (dispersed between 2400 and 800 μm ventral)900 nl2A–C, 3
C57BL/6J2 MRetrobeadsOB200 nl2A–C, 3
C57BL/6J7 MAAVretro-GFPOB200 nl2D, 4A–G
C57BL/6J2 MAAVretro-GFPOT500 nl4H
Ai96 MAAVretro-CreOB200 nl2E, 5A–G
Ai91 M, 1 FAAVretro-CreOT500 nl5H
Ai91 M, 2 sFAAVretro-CreOB (1, 1.5, and 2 mm anterior the rhinal sinus, 1 mm lateral, 1.5 mm ventral)200 nl × 3 sites = 600 nl total6
  • Unless otherwise noted, OB injections were given at 1.5 mm anterior the rhinal sinus, 1 mm lateral, and 1.5 mm ventral, and OT injections were given at 1.5 mm anterior bregma, 1.2 mm lateral, and 4.8 mm ventral.