Table 1

PINK1-interacting proteins identified by IP-MS analysis

Gene nameDescriptionGFP-IP PSMPINK1-GFP -IP PSMLog2 ratio (PINK1-GFP/GFP)
PINK1PTEN-induced kinase 104159.70
CDC37Cell division cycle 37 homolog0476.60
VCPValosin-containing protein0446.49
PSMB5Proteasome subunit, beta type, 50396.30
TIMM50Translocase of inner mitochondrial membrane 50 homolog0225.49
TOMM40Translocase of outer mitochondrial membrane 40 homolog0195.30
GFPGreen fluorescent protein224204–0.13
  • PINK1-interacting proteins identified by a label-free mass spectrometry screen of GFP and PINK1-GFP IP products. The data were SEQUEST searched against the Uniprot human proteome database supplemented with the GFP sequence (P42212). Log2 ratios were calculated as described in Materials and Methods from the total peptide-spectral match counts in PINK1-GFP versus GFP control reactions. Top ranked candidates and known PINK1 interacting proteins are shown, all of which exhibited ratios >2 SD from the mean ratio of the entire dataset.