Antibodies
| Peptide/protein Target | Name of antibody (RRID when available) | Manufacturer, catalog number, and/or name of individual providing the antibody | Species raised in; monoclonal or polyclonal | Dilution used | Tissue; labeling |
|---|---|---|---|---|---|
| rbGnRH | SW-1 (AB_2629221) | S. Wray | Rabbit; polyclonal | 1:3000 | Explants; DAB (Fig. 1A) |
| GIRK2 | APC-006 (AB_2040115) | Alomone Labs | Rabbit; polyclonal | 1:800 | Explants; DAR-488 (Fig. 7) |
| mGnRH | F1D3C5 (n/a) + SMI-41 (AB_10123893) | A. Karande + Abcam | Mouse; monoclonal | 1:4000 +1:6000 | Explants; DAM-555 (Fig. 7) |
| N/OFQ | RA10106 (AB_2737116) | Neuromics | Rabbit; polyclonal | 1:3000 | Sections; DAR-555 (Fig. 8) Sections; DAR-488 (Fig. 8-1A,B; Figs. 9, 10D–F) Sections; DAB (Fig. 8-1C,D) |
| GFP | ab13970 (AB_300798) | Abcam | Chicken; polyclonal | 1:2000 | Sections; DAC-488 (Figs. 8, 10A–C) |
| POMC | DP4 (n/a) | P. Loh | Rabbit; polyclonal | 1:2500 | Sections; avidin-Texas Red (Figs. 9, 10D–F) Sections; DAR-555 (Fig. 10A–C) |
| ckGnRH | Custom-made (n/a) | Aves | Chicken; monoclonal | 1:100 | Sections; DAC-647 (Fig. 10E,F) |
| Neurophysin | RN2 (AB_2751000) | A. Robinson | Rabbit; polyclonal | 1:12000 | Section; DAR-488 (Fig. 8-1B) |
DAB, 3,3´-diaminobenzidine; DAM, donkey anti-mouse; DAR, donkey anti-rabbit; DAC, donkey anti-chicken.