Primer sequences
| Gene (NCBI/GenBank ID) Reference Sequence) | Primers sequences (5´ to 3´) | Annealing temperature | Product size |
|---|---|---|---|
| GnRH-1 (NM_008145.2) | CTG ATG GCC GGC ATT CTA CTG C | 66°C | 220 bp |
| CCA GAG CTC CTC GCA GAT CCC | |||
| Opioid receptor-like 1 (NM_011012.5) | CAT GCC ATG CAG AAC CCA G | 55°C | 209 bp |
| AGG GCT AGC TAC ATG CAC GA |