. Whole-cell recording properties for Figures 1–3
| Mean ± SEM of GnRH whole-cell passive properties from Figure 1 | ||||
|---|---|---|---|---|
| Diestrous PM | Proestrus AM | Proestrus PM | ||
| Rin (MΩ) | 929 ± 42 | 1336 ± 160* | 1034 ± 63 | |
| Capacitance (pF) | 14.7 ± 0.7 | 13.0 ± 0.8 | 14.5 ± 0.7 | |
| Rs (MΩ) | 13.2 ± 0.6 | 13.5 ± 1.0 | 14.6 ± 0.7 | |
| Ihold (pA) | -13.6 ± 4.2 | -0.15 ± 4.7# | -17.4 ± 2.8 | |
| *p < 0.05 vs diestrous PM; #p < 0.01 vs proestrous PM, Tukey’s | ||||
| ANOVA parameters for comparison of GnRH passive properties (Fig. 1) | ||||
| Rin (MΩ) | F(2,33) = 4.84 | |||
| Capacitance (pF) | F(2,33) = 1.32 | |||
| Rs (MΩ) | F(2,33) = 0.951 | |||
| Ihold (pA) | F(2,33) = 5.38 | |||
| Mean ± SEM of GnRH whole-cell passive properties from Figure 2 | ||||
| Diestrous PM | Proestrus PM | |||
| Rin (MΩ) Before TTX During TTX | 1053 ± 88 846 ± 83 | 1113 ± 145 775 ± 91 | ||
| Capacitance (pF) Before TTX During TTX | 15.8 ± 0.8 15.0 ± 0.8 | 13.2 ± 0.9 13.8 ± 1.0 | ||
| Rs (MΩ) Before TTX During TTX | 12.1 ± 0.9 13.3 ± 1.5 | 12.3 ± 0.6 14.7 ± 0.8 | ||
| Ihold (pA) Before TTX During TTX | -16.4 ± 3.5 -25.1 ± 6.3 | -19.4 ± 2.6 -28.5 ± 3.8 | ||
| Two-way repeated measures ANOVA for comparison of GnRH passive properties among groups (Fig. 2) | ||||
| Group | TTX | Group × TTX | ||
| Rin (MΩ) | F(1,9) = 0.001 | F(1,9) = 36.0*** | F(1,9) = 2.1 | |
| Capacitance (pF) | F(1,9) = 2.5 | F(1,9) = 0.1 | F(1,9) = 6.9* | |
| Rs (MΩ) | F(1,9) = 0.4 | F(1,9) = 6.0* | F(1,9) = 0.7 | |
| Ihold (pA) | F(1,9) = 0.3 | F(1,9) = 14.1** | F(1,9) = 0.01 | |
| Mean ± SEM of GnRH whole-cell passive properties from Figure 3 | ||||
| Diestrous PM | Proestrous AM | Proestrous PM | ||
| Rin (MΩ) | 1125 ± 150 | 667 ± 43* | 1361 ± 144 | |
| Capacitance (pF) | 13.7 ± 0.7 | 13.8 ± 0.7 | 12.5 ± 0.8 | |
| Rs (MΩ) | 13.5 ± 0.9 | 11.9 ± 0.9 | 13.5 ± 1.4 | |
| Ihold (pA) | -0.7 ± 5.2 | -2.8 ± 6.3 | -10.1 ± 4.4 | |
| *p < 0.05 vs diestrous PM, Tukey’s | ||||
| ANOVA parameters for comparison of GnRH passive properties (Fig. 3) | ||||
| Rin (MΩ) | F(2,22) = 6.65 | |||
| Capacitance (pF) | F(2,22) = 1.02 | |||
| Rs (MΩ) | F(2,22) = 0.62 | |||
| Ihold (pA) | KW = 3.36 | |||