RT Journal Article
SR Electronic
T1 Electrophysiological Signature Reveals Laminar Structure of the Porcine Hippocampus
JF eneuro
JO eNeuro
FD Society for Neuroscience
SP ENEURO.0102-18.2018
DO 10.1523/ENEURO.0102-18.2018
VO 5
IS 5
A1 Alexandra V. Ulyanova
A1 Paul F. Koch
A1 Carlo Cottone
A1 Michael R. Grovola
A1 Christopher D. Adam
A1 Kevin D. Browne
A1 Maura T. Weber
A1 Robin J. Russo
A1 Kimberly G. Gagnon
A1 Douglas H. Smith
A1 H. Isaac Chen
A1 Victoria E. Johnson
A1 D. Kacy Cullen
A1 John A. Wolf
YR 2018
UL http://www.eneuro.org/content/5/5/ENEURO.0102-18.2018.abstract
AB The hippocampus is integral to working and episodic memory and is a central region of interest in diseases affecting these processes. Pig models are widely used in translational research and may provide an excellent bridge between rodents and nonhuman primates for CNS disease models because of their gyrencephalic neuroanatomy and significant white matter composition. However, the laminar structure of the pig hippocampus has not been well characterized. Therefore, we histologically characterized the dorsal hippocampus of Yucatan miniature pigs and quantified the cytoarchitecture of the hippocampal layers. We then utilized stereotaxis combined with single-unit electrophysiological mapping to precisely place multichannel laminar silicon probes into the dorsal hippocampus without the need for image guidance. We used in vivo electrophysiological recordings of simultaneous laminar field potentials and single-unit activity in multiple layers of the dorsal hippocampus to physiologically identify and quantify these layers under anesthesia. Consistent with previous reports, we found the porcine hippocampus to have the expected archicortical laminar structure, with some anatomical and histological features comparable to the rodent and others to the primate hippocampus. Importantly, we found these distinct features to be reflected in the laminar electrophysiology. This characterization, as well as our electrophysiology-based methodology targeting the porcine hippocampal lamina combined with high-channel-count silicon probes, will allow for analysis of spike-field interactions during normal and disease states in both anesthetized and future awake behaving neurophysiology in this large animal.