RT Journal Article SR Electronic T1 The Nogo Receptor Ligand LGI1 Regulates Synapse Number and Synaptic Activity in Hippocampal and Cortical Neurons JF eneuro JO eNeuro FD Society for Neuroscience SP ENEURO.0185-18.2018 DO 10.1523/ENEURO.0185-18.2018 VO 5 IS 4 A1 Rhalena A. Thomas A1 Julien Gibon A1 Carol X. Q. Chen A1 Sabrina Chierzi A1 Vincent G. Soubannier A1 Stephanie Baulac A1 Philippe Séguéla A1 Keith Murai A1 Philip A. Barker YR 2018 UL http://www.eneuro.org/content/5/4/ENEURO.0185-18.2018.abstract AB Leucine-rich glioma-inactivated protein 1 (LGI1) is a secreted neuronal protein and a Nogo receptor 1 (NgR1) ligand. Mutations in LGI1 in humans causes autosomal dominant lateral temporal lobe epilepsy and homozygous deletion of LGI1 in mice results in severe epileptic seizures that cause early postnatal death. NgR1 plays an important role in the development of CNS synapses and circuitry by limiting plasticity in the adult cortex via the activation of RhoA. These relationships and functions prompted us to examine the effect of LGI1 on synapse formation in vitro and in vivo. We report that application of LGI1 increases synaptic density in neuronal culture and that LGI1 null hippocampus has fewer dendritic mushroom spines than in wild-type (WT) littermates. Further, our electrophysiological investigations demonstrate that LGI1 null hippocampal neurons possess fewer and weaker synapses. RhoA activity is significantly increased in cortical cultures derived from LGI1 null mice and using a reconstituted system; we show directly that LGI1 antagonizes NgR1-tumor necrosis factor receptor orphan Y (TROY) signaling. Our data suggests that LGI1 enhances synapse formation in cortical and hippocampal neurons by reducing NgR1 signaling.