TY - JOUR T1 - Rostrocaudal Areal Patterning of Human PSC-Derived Cortical Neurons by FGF8 Signaling JF - eneuro JO - eNeuro DO - 10.1523/ENEURO.0368-17.2018 VL - 5 IS - 2 SP - ENEURO.0368-17.2018 AU - Kent Imaizumi AU - Koki Fujimori AU - Seiji Ishii AU - Asako Otomo AU - Yasushi Hosoi AU - Hiroaki Miyajima AU - Hitoshi Warita AU - Masashi Aoki AU - Shinji Hadano AU - Wado Akamatsu AU - Hideyuki Okano Y1 - 2018/03/01 UR - http://www.eneuro.org/content/5/2/ENEURO.0368-17.2018.abstract N2 - The cerebral cortex is subdivided into distinct areas that have particular functions. The rostrocaudal (R-C) gradient of fibroblast growth factor 8 (FGF8) signaling defines this areal identity during neural development. In this study, we recapitulated cortical R-C patterning in human pluripotent stem cell (PSC) cultures. Modulation of FGF8 signaling appropriately regulated the R-C markers, and the patterns of global gene expression resembled those of the corresponding areas of human fetal brains. Furthermore, we demonstrated the utility of this culture system in modeling the area-specific forebrain phenotypes [presumptive upper motor neuron (UMN) phenotypes] of amyotrophic lateral sclerosis (ALS). We anticipate that our culture system will contribute to studies of human neurodevelopment and neurological disease modeling. ER -