@article {LiuENEURO.0259-17.2017, author = {Xinhuai Liu and Robert Porteous and Allan E. Herbison}, title = {Dynamics of GnRH Neuron Ionotropic GABA and Glutamate Synaptic Receptors Are Unchanged during Estrogen Positive and Negative Feedback in Female Mice}, volume = {4}, number = {5}, elocation-id = {ENEURO.0259-17.2017}, year = {2017}, doi = {10.1523/ENEURO.0259-17.2017}, publisher = {Society for Neuroscience}, abstract = {Inputs from GABAergic and glutamatergic neurons are suspected to play an important role in regulating the activity of the gonadotropin-releasing hormone (GnRH) neurons. The GnRH neurons exhibit marked plasticity to control the ovarian cycle with circulating estradiol concentrations having profound {\textquotedblleft}feedback{\textquotedblright} effects on their activity. This includes {\textquotedblleft}negative feedback{\textquotedblright} responsible for suppressing GnRH neuron activity and {\textquotedblleft}positive feedback{\textquotedblright} that occurs at mid-cycle to activate the GnRH neurons to generate the preovulatory luteinizing hormone surge. In the present study, we employed brain slice electrophysiology to question whether synaptic ionotropic GABA and glutamate receptor signaling at the GnRH neuron changed at times of negative and positive feedback. We used a well characterized estradiol (E){\textendash}treated ovariectomized (OVX) mouse model to replicate negative and positive feedback. Miniature and spontaneous postsynaptic currents (mPSCs and sPSCs) attributable to GABAA and glutamatergic receptor signaling were recorded from GnRH neurons obtained from intact diestrous, OVX, OVX + E (negative feedback), and OVX + E+E (positive feedback) female mice. Approximately 90\% of GnRH neurons exhibited spontaneous GABAA-mPSCs in all groups but no significant differences in the frequency or kinetics of mPSCs were found at the times of negative or positive feedback. Approximately 50\% of GnRH neurons exhibited spontaneous glutamate mPSCs but again no differences were detected. The same was true for spontaneous PSCs in all cases. These observations indicate that the kinetics of ionotropic GABA and glutamate receptor synaptic transmission to GnRH neurons remain stable across the different estrogen feedback states.}, URL = {https://www.eneuro.org/content/4/5/ENEURO.0259-17.2017}, eprint = {https://www.eneuro.org/content/4/5/ENEURO.0259-17.2017.full.pdf}, journal = {eNeuro} }