TY - JOUR T1 - Mesocortical Dopamine Phenotypes in Mice Lacking the Sonic Hedgehog Receptor Cdon JF - eneuro JO - eneuro DO - 10.1523/ENEURO.0009-16.2016 SP - ENEURO.0009-16.2016 AU - Michael Verwey AU - Alanna Grant AU - Nicholas Meti AU - Lauren Adye-White AU - Angelica Torres-Berrío AU - Veronique Rioux AU - Martin Lévesque AU - Frederic Charron AU - Cecilia Flores Y1 - 2016/06/29 UR - http://www.eneuro.org/content/early/2016/06/29/ENEURO.0009-16.2016.abstract N2 - Motivated behaviors and many psychopathologies typically involve changes in dopamine release from the projections of the ventral tegmental area (VTA) and/or the substantia nigra pars compacta (SNc). The morphogen Sonic Hedgehog (Shh) specifies fates of midbrain dopamine neurons, but VTA-specific effects of Shh signaling are also being uncovered. In this study we assessed the role of the Shh receptor Cdon in the development of VTA and SNc dopamine neurons. We find that Cdon is expressed in the proliferating progenitor zone of the embryonic ventral midbrain and that the number of proliferating cells in this region is increased in mouse Cdon-/- embryos. Consistent with a role of Shh in the regulation of neuronal proliferation in this region, we find that the number of TH-positive neurons is increased in the VTA of Cdon-/- mice at birth and that this effect endures into adulthood. In contrast, the number of TH-positive neurons in the SNc is not altered in Cdon-/- mice at either age. Moreover, adult Cdon-/- mice have a greater number of medial prefrontal cortex (mPFC) dopamine presynaptic sites, and increased baseline concentrations of dopamine and dopamine metabolites selectively in this region. Finally, consistent with increased dopamine function in the mPFC, we find that adult Cdon-/- mice fail to exhibit behavioral plasticity upon repeated amphetamine treatment. Based on these data, we suggest that Cdon plays an important role encoding the diversity of dopamine neurons in the midbrain, influencing both the development of the mesocortical dopamine pathway and behavioral outputs that involve this neural circuitry.Significance Statement: Sonic hedgehog signaling is involved in the specification and development of dopamine neurons in the ventral midbrain. Here we demonstrate that the Shh receptor, Cdon, plays a role in the development of dopamine neurons in the ventral tegmental area. Moreover, this effect of Cdon is selective to the dopamine neurons that project to the medial prefrontal cortex. Adult mice that lack Cdon also fail to show amphetamine-induced behavioral plasticity. Our findings show that the Cdon receptor is important in encoding the diversity of dopamine neurons in the midbrain, influencing both the development of the mesocortical dopamine pathway as well as behavioral outputs that involve this neural circuitry. ER -