@article {Perez-PouchoulenENEURO.0036-15.2015, author = {Miguel Perez-Pouchoulen and Jonathan W. VanRyzin and Margaret M. McCarthy}, title = {Morphological and Phagocytic Profile of Microglia in the Developing Rat Cerebellum}, volume = {2}, number = {4}, elocation-id = {ENEURO.0036-15.2015}, year = {2015}, doi = {10.1523/ENEURO.0036-15.2015}, publisher = {Society for Neuroscience}, abstract = {Microglia are being increasingly recognized as playing important roles in neurodevelopment. The cerebellum matures postnatally, undergoing major growth, but the role of microglia in the developing cerebellum is not well understood. Using the laboratory rat we quantified and morphologically categorized microglia throughout the vermis and across development using a design-based unbiased stereology method. We found that microglial morphology changed from amoeboid to ramified during the first 3 postnatal weeks in a region specific manner. These morphological changes were accompanied by the sudden appearance of phagocytic cups during the third postnatal week from P17 to P19, with an approximately fourfold increase compared with the first week, followed by a prompt decline at the end of the third week. The microglial phagocytic cups were significantly higher in the granular layer (\~{}69\%) than in the molecular layer (ML; \~{}31\%) during a 3 d window, and present on \~{}67\% of microglia with thick processes and \~{}33\% of microglia with thin processes. Similar proportions of phagocytic cups associated to microglia with either thick or thin processes were found in the ML. We observed cell nuclei fragmentation and cleaved caspase-3 expression within some microglial phagocytic cups, presumably from dying granule neurons. At P17 males showed an approximately twofold increase in microglia with thin processes compared with females. Our findings indicate a continuous process of microglial maturation and a nonuniform distribution of microglia in the cerebellar cortex that implicates microglia as an important cellular component of the developing cerebellum.}, URL = {https://www.eneuro.org/content/2/4/ENEURO.0036-15.2015}, eprint = {https://www.eneuro.org/content/2/4/ENEURO.0036-15.2015.full.pdf}, journal = {eNeuro} }