TY - JOUR T1 - Neuronal Adenosine A2A Receptors Are Critical Mediators of Neurodegeneration Triggered by Convulsions JF - eneuro JO - eNeuro DO - 10.1523/ENEURO.0385-18.2018 VL - 5 IS - 6 SP - ENEURO.0385-18.2018 AU - Paula M. Canas AU - Lisiane O. Porciúncula AU - Ana Patrícia Simões AU - Elisabete Augusto AU - Henrique B. Silva AU - Nuno J. Machado AU - Nélio Gonçalves AU - Tiago M. Alfaro AU - Francisco Q. Gonçalves AU - Inês M. Araújo AU - Joana I. Real AU - Joana E. Coelho AU - Geanne M. Andrade AU - Ramiro D. Almeida AU - Jiang-Fan Chen AU - Attila Köfalvi AU - Paula Agostinho AU - Rodrigo A. Cunha Y1 - 2018/11/01 UR - http://www.eneuro.org/content/5/6/ENEURO.0385-18.2018.abstract N2 - Neurodegeneration is a process transversal to neuropsychiatric diseases and the understanding of its mechanisms should allow devising strategies to prevent this irreversible step in brain diseases. Neurodegeneration caused by seizures is a critical step in the aggravation of temporal lobe epilepsy, but its mechanisms remain undetermined. Convulsions trigger an elevation of extracellular adenosine and upregulate adenosine A2A receptors (A2AR), which have been associated with the control of neurodegenerative diseases. Using the rat and mouse kainate model of temporal lobe epilepsy, we now tested whether A2AR control convulsions-induced hippocampal neurodegeneration. The pharmacological or genetic blockade of A2AR did not affect kainate-induced convulsions but dampened the subsequent neurotoxicity. This neurotoxicity began with a rapid A2AR upregulation within glutamatergic synapses (within 2 h), through local translation of synaptic A2AR mRNA. This bolstered A2AR-mediated facilitation of glutamate release and of long-term potentiation (LTP) in CA1 synapses (4 h), triggered a subsequent synaptotoxicity, heralded by decreased synaptic plasticity and loss of synaptic markers coupled to calpain activation (12 h), that predated overt neuronal loss (24 h). All modifications were prevented by the deletion of A2AR selectively in forebrain neurons. This shows that synaptic A2AR critically control synaptic excitotoxicity, which underlies the development of convulsions-induced neurodegeneration. ER -