PT - JOURNAL ARTICLE AU - Nadine Thiele AU - Christine Köppl TI - Gas Anesthesia Impairs Peripheral Auditory Sensitivity in Barn Owls (<em>Tyto alba</em>) AID - 10.1523/ENEURO.0140-18.2018 DP - 2018 Sep 01 TA - eneuro PG - ENEURO.0140-18.2018 VI - 5 IP - 5 4099 - http://www.eneuro.org/content/5/5/ENEURO.0140-18.2018.short 4100 - http://www.eneuro.org/content/5/5/ENEURO.0140-18.2018.full SO - eNeuro2018 Sep 01; 5 AB - Auditory nerve single-unit recordings were obtained from two groups of young barn owls (age, between posthatching days 11 and 86) in terminal experiments under two different anesthetic regimes: ketamine (6–11 mg/kg) plus xylazine (∼2 mg/kg); or isoflurane (1–1.5%) in oxygen, delivered via artificial respiration. In a second series of minimally invasive experiments, auditory brainstem responses (ABRs) were recorded in the same four adult barn owls (Tyto alba; age, between 5 and 32 months) under three different anesthetic protocols: ketamine (10 mg/kg) plus xylazine (3 mg/kg), isoflurane (1–1.5%), and sevoflurane (2–3%) in carbogen. Finally, the ABR measurements on adult owls were repeated in terminal experiments including more invasive procedures such as artificial respiration and higher isoflurane dosage. The main finding was a significant deterioration of auditory sensitivity in barn owls under gas anesthesia, at the level of the auditory nerve (i.e., a very peripheral level of the auditory system). The effect was drastic in the young animals that experienced threshold elevations in auditory nerve single-unit responses of ≥20 dB. ABR thresholds assessed repeatedly in experiments on adult owls were also significantly higher under isoflurane and sevoflurane, on average by 7 and 15 dB, compared with ketamine/xylazine. This difference already occurred with minimal dosages and was reversibly enlarged with increased isoflurane concentration. Finally, there was evidence for confounding detrimental effects associated with artificial respiration over many hours, which suggested oxygen toxicity.