Figure 3. Transcriptome comparison between PSC-derived neural progenitors and human fetal brains. A, Volcano plot of the expression profile of FGF8-treated cells relative to control, with differentially expressed genes (p < 0.05; Student’s t test) highlighted (dark gray). The rostral (red) and caudal (blue) markers were enriched in FGF8-treated and untreated cells, respectively. B, C, Correlation matrix of the global gene expression with macro-dissected (B) and micro-dissected (C) human fetal brains. FGF8-treated cells well correlated with the rostral portions of macro-dissected brains and with the sensorimotor cortex of micro-dissected brains. OFC, orbital prefrontal cortex; MFC, mediolateral prefrontal cortex; DFC, dorsolateral prefrontal cortex; VFC, ventrolateral prefrontal cortex; M1C, primary motor cortex; S1C, primary sensory cortex; IPC, inferior parietal cortex; A1C, primary auditory cortex; STC, superior temporal cortex; ITC, inferolateral temporal cortex; V1C, primary visual cortex.