Figure 3. Effects of 5d-RFSS on depressive-like behavior in BALB/cJ mice. A, Immobility time of 5d-RFSS BALB/cJ mice during the induction phase (days 1–5) of forced swim tests (FST; main effect of time, F(4,36) = 6.29, p = 0.0006; post hoc test, §p = 0.02, §§p = 0.002 vs day 1) and of 5d-RFSS and nonstressed control mice during the test phase (day 37; t(1,18) = 3.08, *p = 0.007). B, Two-bottle SPTs before 5d-RFSS (SPT1) and after 5d-RFSS (SPT2 to SPT4; main effect of time, F(3,54) = 12.67, p < 0.00001; post hoc test, §p = 0.002, §§p = 0.0002 vs SPT1). C, TST immobility time before 5d-RFSS (TST1) and after 5d-RFSS (TST2; main effect of time, F(1,18) = 5.06, p = 0.04; post hoc test, §p = 0.04 vs TST1). D–F, Distance traveled (t(1,18) = 0.74, p = 0.47; D), number of center zone entries (E), and number of feces produced during the OFT (t(1,18) = 0.79, p = 0.44; F). G, Change in body weight (%) from days −3 to 36 (main effect of time, F(6,108) = 36.96, p < 0.00001; post hoc test, §p = 0.002, §§p = 0.0001 vs day −3). H, I, Food intake during the induction phase (days 1 – 5; t(1,18) = 0.07, p = 0.94; H) and during days 8–36 (t(1,18) = 1.36, p = 0.19; I). NS, Not significant; ND, not detected. n = 10/group for all experiments.