Figure 9. TFEB expression in the P301S mice significantly attenuates learning and memory deficits. A, In the Barnes maze test, on day 1 of training, time spent in the target quadrant was 33% less by P301S mice and only 18% less by P301S/flag-TFEB double-transgenic mice compared with WT controls. On day 2, P301S mice spent 32% less time, but double-transgenic mice spent only 5% less time. On day 4, the difference was 38% for P301S mice and only 6% for the double-transgenic mice. During the probe test, the difference was from 50% to 17%. B, In the T maze paradigm, on day 3 of testing, the correct responses were 58% for WT mice, 28% for P301S mice, and 57% for double-transgenic mice. On day 4 of testing, the correct responses were 73% for WT mice, 43% for P301S mice, and 68% for double-transgenic mice. On day 5, the correct responses were 87% for WT mice, 55% for P301S mice and 80% for the double-transgenic mice. During the probe test, the correct responses were 82% for WT mice, 32% for P301S mice, and 68% for the double-transgenic mice. The latency also differed. On day 3, the average latencies for WT, P301S, and double-transgenic mice were 29, 45, and 36 s, respectively. On day 5, the average latencies for WT, P301S, and double-transgenic mice were 17, 32, and 19 s, respectively. On the day of the probe test, the latencies for WT, P301S, and double-transgenic mice were 14, 30, and 18 s, respectively. *p < 0.05, **p < 0.01, and ***p < 0.001, compared with WT controls. $$p < 0.01, $$$p < 0.001, compared with P301S mice. The data are reported as the mean ± SEM. n = 6 per genotype.